Anti-NF-kB p65 (phospho S468) antibody (ab31473)
Key features and details
- Rabbit polyclonal to NF-kB p65 (phospho S468)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NF-kB p65 (phospho S468) antibody
See all NF-kB p65 primary antibodies -
Description
Rabbit polyclonal to NF-kB p65 (phospho S468) -
Host species
Rabbit -
Specificity
This antibody detects endogenous levels of NF-kB p65 protein only when phosphorylated at Serine 468. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human NF-kB p65 aa 435-484.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
This antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF-kB p65 (phospho S468) antibody (ab31473)
Ab31473, at a dilution of 1/50, staining NF-kB p65 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
Left image: Untreated.
Right image: Treated with synthesized peptide. -
Western blot - Anti-NF-kB p65 (phospho S468) antibody (ab31473)All lanes : Anti-NF-kB p65 (phospho S468) antibody (ab31473) at 1/500 dilution
Lane 1 : Extracts from MOLT cells (5-30ug).
Lane 2 : Extracts from ovary cancer cells (5-30ug).
Lane 3 : Extracts from Hela cells (5-30ug).
Secondary
All lanes : goat-anti-rabbit IgG-AP-conjugate.
Predicted band size: 60 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Additional bands at: 42 kDa. We are unsure as to the identity of these extra bands.
