Anti-Nck-2 antibody [EPR3333] (ab109239)
![Anti-Nck-2 antibody [EPR3333] (ab109239)](https://www.abcam.com/ps/products/109/ab109239/Images/ab109239-121918-anti-nck-2-antibody-epr3333-western-blot.jpg "Anti-Nck-2 antibody [EPR3333] (ab109239)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3333] to Nck-2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-Nck-2 antibody [EPR3333]
See all Nck-2 primary antibodies -
Description
Rabbit monoclonal [EPR3333] to Nck-2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human spleen lysate, human fetal brain lysate, SH-SY5Y lysate, Human fetal kidney lysate; Human breast ductal carcinoma tissue; HeLa cells
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General notes
Protein previously labeled as Nck beta.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3333 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Nck-2 antibody [EPR3333] (ab109239)All lanes : Anti-Nck-2 antibody [EPR3333] (ab109239) at 1/1000 dilution
Lane 1 : Human spleen lysate
Lane 2 : Human fetal brain lysate
Lane 3 : SH-SY5Y lysate
Lane 4 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 43 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nck-2 antibody [EPR3333] (ab109239)](https://www.abcam.com/ps/products/109/ab109239/Images/ab109239-121920-anti-nck-2-antibody-epr3333-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nck-2 antibody [EPR3333] (ab109239)Immunohistochemical analysis of paraffin-embedded human breast ductal carcinoma tissue using ab109239 at a dilution of 1/50
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Nck-2 antibody [EPR3333] (ab109239)](https://www.abcam.com/ps/products/109/ab109239/Images/ab109239-121919-anti-nck-2-antibody-epr3333-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Nck-2 antibody [EPR3333] (ab109239)Immunofluorescent staining of HeLa cells using ab109239 at a dilution of 1/100 -
![Anti-Nck-2 antibody [EPR3333] (ab109239)](https://www.abcam.com/ps/products/109/ab109239/Images/ab109239-4-benefits-of-recombinant-antibodies.png)
Anti-Nck-2 antibody [EPR3333] (ab109239)
