Anti-N Cadherin (phospho Y820) antibody (ab76059)
Key features and details
- Rabbit polyclonal to N Cadherin (phospho Y820)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-N Cadherin (phospho Y820) antibody
See all N Cadherin primary antibodies -
Description
Rabbit polyclonal to N Cadherin (phospho Y820) -
Host species
Rabbit -
Specificity
ab76059 detects a 130 kDa band corresponding to N cadherin in western blots of serum-starved human endothelial cells treated with pervanadate, but it is not detected in untreated cells. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic phospho-peptide (coupled to carrier protein) corresponding to amino acids surrounding tyrosine 820 in human N cadherin.
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Positive control
- Human endothelial cells treated with pervanadate (1 mM) for 30 min.
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General notes
Do not aliquot this antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab76059 was cross-adsorbed to phospho-N Cadherin (Tyr-860) and unphosphorylated N cadherin (Tyr-820) peptides before affinity purification using phospho-N cadherin (Tyr-820) peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-N Cadherin (phospho Y820) antibody (ab76059)All lanes : Anti-N Cadherin (phospho Y820) antibody (ab76059) at 1/1000 dilution
Lane 1 : Human endothelial cells untreated
Lane 2 : Human endothelial cells treated with pervanadate (1 mM) for 30 min
Lane 3 : Human endothelial cells treated with pervanadate (1 mM) for 30 min with phospho-N-cadherin (Tyr-820) peptide
Lane 4 : Human endothelial cells treated with pervanadate (1 mM) for 30 min with phospho-N-cadherin (Tyr-860) peptide
Predicted band size: 100 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
