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Human YB1 Antibody Pair - BSA and Azide free (ab270381)

Key features and details

  • Unconjugated capture and detector antibodies
  • Adaptable to any antibody pair-based assay format
  • Antibody concentration ~ 1 mg/ml
  • BSA and azide free buffer - ready for conjugation
  • Reacts with: Human

Overview

  • Product name

    Human YB1 Antibody Pair - BSA and Azide free
    See all YB1 kits

  • Assay type

    ELISA set

  • Range

    0.938 pg/ml - 60 pg/ml

  • Species reactivity

    Reacts with: Human

  • Product overview

    The Antibody Pair can be used to quantify Human YB1. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


    For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab269544), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


    Download SDS here.

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.

  • Carrier free

    Yes
  • Components 10 x 96 tests
    Human YB1 Capture Antibody (unconjugated) 1 x 100µg
    Human YB1 Detector Antibody (unconjugated) 1 x 100µg

  • Research areas

  • Function

    Mediates pre-mRNA alternative splicing regulation. Binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors (By similarity). Regulates the transcription of numerous genes. Its transcriptional activity on the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7'. Binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as MDR1 and HLA class II genes. Promotes separation of DNA strands that contain mismatches or are modified by cisplatin. Has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro). May play a role in DNA repair. Component of the CRD-mediated complex that promotes MYC mRNA stability.
    The secreted form acts as an extracellular mitogen and stimulates cell migration and proliferation.

  • Sequence similarities

    Contains 1 CSD (cold-shock) domain.

  • Post-translational
    modifications

    Ubiquitinated by RBBP6; leading to a decrease of YBX1 transcativational ability.
    In the absence of phosphorylation the protein is retained in the cytoplasm.
    Cleaved by a 20S proteasomal protease in response to agents that damage DNA. Cleavage takes place in the absence of ubiquitination and ATP. The resulting N-terminal fragment accumulates in the nucleus.

  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasmic granule. Secreted. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles between nucleus and cytoplasm. Predominantly cytoplasmic in proliferating cells. Cytotoxic stress and DNA damage enhance translocation to the nucleus. Localized with DDX1, MBNL1 and TIAL1 in stress granules upon stress. Secreted by mesangial and monocytic cells after inflammatory challenges. Translocates from the cytoplasm to the nucleus after and colocalizes with APEX1 in nuclear speckles after genotoxic stress.
  • Information by UniProt

  • Alternative names

    • BP 8
    • CBF-A
    • CCAAT binding transcription factor I subunit A
    • CCAAT-binding transcription factor I subunit A
    • CSDA2
    • CSDB
    • DBPB
    • DNA binding protein B
    • DNA-binding protein B
    • EFI-A
    • Enhancer factor I subunit A
    • MDR NF1
    • MGC104858
    • MGC110976
    • MGC117250
    • NSEP 1
    • NSEP1
    • Nuclease sensitive element binding protein 1
    • Nuclease-sensitive element-binding protein 1
    • p50
    • Q15905
    • Y-box binding protein 1
    • Y-box transcription factor
    • Y-box-binding protein 1
    • YB 1
    • YB-1
    • YBOX1_HUMAN
    • YBX 1
    • ybx1
    see all

  • Database links

    Images

    • Human YB1 Antibody Pair - BSA and Azide free (ab270381)
      Human YB1 Antibody Pair - BSA and Azide free (ab270381)

      To learn more about the advantages of recombinant antibodies see here.

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