Anti-Myosin VIIa/MYO7A antibody (ab3481)
Key features and details
- Rabbit polyclonal to Myosin VIIa/MYO7A
- Suitable for: WB, ICC
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
-
Product name
Anti-Myosin VIIa/MYO7A antibody
See all Myosin VIIa/MYO7A primary antibodies -
Description
Rabbit polyclonal to Myosin VIIa/MYO7A -
Host species
Rabbit -
Specificity
Detects Myosin VIIa/MYO7A from mouse tissues as well as recombinant. By Western blot, this antibody detects an ~220 kDa protein representing myosin VIIa/MYO7A from mouse testes preparations. This antibody detects recombinant mouse myosin VIIa/MYO7A overexpressed in Sf9 insect cell lysate.
-
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Mouse Myosin VIIa/MYO7A aa 16-31.
Sequence:SGQEFDVPIGAVVKLC
(Peptide available asab4996) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Primary antibody notes
Myosin VIIa is a member of the myosin superfamily of actin-based motor proteins. Defects in the myosin VIIa gene are responsible for hearing impairment in shaker-1 (sh1) mice and causes Usher syndrome IB in humans. Usher syndrome associates congenital deafness, vestibular dysfunction, and retinitis pigmentosa and is the most common form of combined deafness and blindness. Structural features of myosin VIIa protein include an ATP binding N-terminal motor domain, a central region which possess five light-chain binding (IQ) motifs, and a C-terminal domain with three myosin tail homology (MyTH4) and talin-like homology regions. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Myosin VIIa/MYO7A antibody (ab3481)Anti-Myosin VIIa/MYO7A antibody (ab3481) at 5 µg/ml + Membrane enriched extracts of L6 at 30 µg with Skimmed milk at 5 %
Secondary
HRP conjugated goat anti-rabbit IgG (H+L) at 1/4000 dilution
-
Western blot - Anti-Myosin VIIa/MYO7A antibody (ab3481)Western blot detection of Myosin VIIa/MYO7A in mouse testes tissue extract using ab3481.
-
Flow Cytometry - Anti-Myosin VIIa/MYO7A antibody (ab3481)ab3481 staining Myosin VIIa/MYO7A (red histogram) on L6 cells by Flow Cytometry. Cells were fixed with 70% ethonal, permeabilized with Triton and blocked with 5% BSA for 30 minutes at room temperature. The sample was incubated with the primary antibody (3-5 ug/10^6 in 2.5% BSA) for 2 hours at room temperature. An Alexa fluor® 488-conjugated Goat anti-rabbit IgG (1/400) was used as the secondary antibody. Purple histogram represents unstained control, pink histogram represents rabbit isotype control and green histogram represents no primary antibody control
