Antibodies, Proteins, Kits and Reagents for Life Science

Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (ab251338)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17322] to MYOM1 - BSA and Azide free
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-MYOM1 antibody [EPR17322] - BSA and Azide free
See all MYOM1 primary antibodies
Description

Rabbit monoclonal [EPR17322] to MYOM1 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-Pmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
General notes
Ab251338 is the carrier-free version of ab201228. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251338 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Clonality

Monoclonal
Clone number

EPR17322
Isotype

IgG
Research areas

Western blot - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (ab251338)

All lanes : Anti-MYOM1 antibody [EPR17322] (ab201228) at 1/20000 dilution

Lane 1 : Mouse heart lysate
Lane 2 : Mouse muscle lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 188 kDa
Observed band size: 188 kDa

Exposure time: 1 minute

This data was developed using ab201228, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (ab251338)

All lanes : Anti-MYOM1 antibody [EPR17322] (ab201228) at 1/20000 dilution

Lane 1 : Rat heart lysate
Lane 2 : Rat brain lysate
Lane 3 : Rat kidney lysate
Lane 4 : C6 (Rat glial tumor cells) whole cell lysate
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 188 kDa
Observed band size: 188 kDa

Exposure time: 15 seconds

This data was developed using ab201228, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (ab251338)

Anti-MYOM1 antibody [EPR17322] (ab201228) at 1/20000 dilution + Human fetal heart tissue lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 188 kDa
Observed band size: 188 kDa

Exposure time: 3 minutes

This data was developed using ab201228, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (ab251338)

This data was developed using ab201228, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling MYOM1 with ab201228 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on Human skeletal muscle tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (ab251338)

This data was developed using ab201228, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MYOM1 with ab201228 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Human tonsil tissue is a negative control for MYOM1. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (ab251338)

This data was developed using ab201228, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling MYOM1 with ab201228 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on mouse skeletal muscle tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (ab251338)

This data was developed using ab201228, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling MYOM1 with ab201228 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on rat skeletal muscle tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (ab251338)

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