Anti-Fast Myosin Skeletal Heavy chain + MYH4 antibody [EPR22880-64] (ab221149)
![Anti-Fast Myosin Skeletal Heavy chain + MYH4 antibody [EPR22880-64] (ab221149)](https://www.abcam.com/ps/products/221/ab221149/Images/ab221149-3-anti-myh1-myh4-antibody-epr22880-64-immunohistochemistry-skeletal-muscle-human.jpg "Anti-Fast Myosin Skeletal Heavy chain + MYH4 antibody [EPR22880-64] (ab221149)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22880-64] to Fast Myosin Skeletal Heavy chain + MYH4
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Fast Myosin Skeletal Heavy chain + MYH4 antibody [EPR22880-64] -
Description
Rabbit monoclonal [EPR22880-64] to Fast Myosin Skeletal Heavy chain + MYH4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human skeletal muscle tissue lysate. IHC-P: Human skeletal muscle tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22880-64 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fast Myosin Skeletal Heavy chain + MYH4 antibody [EPR22880-64] (ab221149)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Fast Myosin Skeletal Heavy chain + MYH4 with ab221149 at 1/1000 dilution (0.505 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human skeletal muscle (PMID: 30274168, 25217814).
The section was incubated with ab221149 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) .
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fast Myosin Skeletal Heavy chain + MYH4 antibody [EPR22880-64] (ab221149)](https://www.abcam.com/ps/products/221/ab221149/Images/ab221149-2-anti-myh1-myh4-antibody-epr22880-64-immunohistochemistry-liver-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fast Myosin Skeletal Heavy chain + MYH4 antibody [EPR22880-64] (ab221149)Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Fast Myosin Skeletal Heavy chain + MYH4 with ab221149 at 1/1000 dilution (0.505 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining on human liver (PMID: 30274168). The section was incubated with ab221149 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) .
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![Western blot - Anti-Fast Myosin Skeletal Heavy chain + MYH4 antibody [EPR22880-64] (ab221149)](https://www.abcam.com/ps/products/221/ab221149/Images/ab221149-1-anti-myh1-myh4-antibody-epr22880-64-western-blot-skeletal-muscle-human.jpg)
Western blot - Anti-Fast Myosin Skeletal Heavy chain + MYH4 antibody [EPR22880-64] (ab221149)Anti-Fast Myosin Skeletal Heavy chain + MYH4 antibody [EPR22880-64] (ab221149) at 1/1000 dilution + Human skeletal muscle tissue lysate at 20 µg
Secondary
VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 223 kDa
Exposure time: 37 secondsBlocking/diluting buffer and concentration: 5% NFDM/TBST.
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![Anti-Fast Myosin Skeletal Heavy chain + MYH4 antibody [EPR22880-64] (ab221149)](https://www.abcam.com/ps/products/221/ab221149/Images/ab221149-4-benefits-of-recombinant-antibodies.png)
Anti-Fast Myosin Skeletal Heavy chain + MYH4 antibody [EPR22880-64] (ab221149)