Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MYH7B antibody [EPR12290] (ab172967)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12290] to MYH7B
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MYH7B antibody [EPR12290]
    See all MYH7B primary antibodies

  • Description

    Rabbit monoclonal [EPR12290] to MYH7B

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    within Human MYH7B. The exact sequence is proprietary.
    Database link: A7E2Y1

  • Positive control

    • WB: Human fetal muscle, fetal heart and skeletal muscle lysates. IHC-P: Human skeletal muscle, cardiac muscle, pancreas and heart tissues. ICC/IF: Rat glial tumor cell line.

  • General notes

    The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR12290

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (ab172967)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (ab172967)

    Immunohistochemical analysis of paraffin embedded Human heart tissue labeling MYH7B with ab172967 at a 1/250 dilution.

  • Western blot - Anti-MYH7B antibody [EPR12290] (ab172967)
    Western blot - Anti-MYH7B antibody [EPR12290] (ab172967)
    Anti-MYH7B antibody [EPR12290] (ab172967) at 0.05 µg/ml (Purified) + Human fetal muscle lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 221 kDa
    Observed band size: 221 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (ab172967)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (ab172967)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cardiac muscle tissue sections labeling MYH7B with Purified ab172967 at 1:1000 dilution (1.03 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (ab172967)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (ab172967)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skeletal muscle tissue sections labeling MYH7B with Purified ab172967 at 1:1000 dilution (1.03 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.

  • Western blot - Anti-MYH7B antibody [EPR12290] (ab172967)
    Western blot - Anti-MYH7B antibody [EPR12290] (ab172967)
    All lanes : Anti-MYH7B antibody [EPR12290] (ab172967) at 0.1 µg/ml (Purified)

    Lane 1 : Mouse heart lysate
    Lane 2 : Rat heart lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 221 kDa
    Observed band size: 221 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (ab172967)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (ab172967)

    ab172967 showing +ve staining in Human normal pancreas tissue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (ab172967)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (ab172967)

    Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue labeling MYH7B with ab172967 at a 1/250 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (ab172967)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (ab172967)
    ab172967 showing -ve staining in Human normal liver tissue.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (ab172967)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (ab172967)
    ab172967 showing -ve staining in Human normal breast tissue.
  • Western blot - Anti-MYH7B antibody [EPR12290] (ab172967)
    Western blot - Anti-MYH7B antibody [EPR12290] (ab172967)
    All lanes : Anti-MYH7B antibody [EPR12290] (ab172967) at 1/1000 dilution (unpurified)

    Lane 1 : Human fetal muscle lysates
    Lane 2 : Human fetal heart lysates
    Lane 3 : Human skeletal muscle lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 221 kDa

  • Anti-MYH7B antibody [EPR12290] (ab172967)
    Anti-MYH7B antibody [EPR12290] (ab172967)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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