Antibodies, Proteins, Kits and Reagents for Life Science

335 040 ₸ Product size

100 µl 335 040 ₸ 10 µl 140 716 ₸

Order now and get it on Tuesday March 02, 2021

Anti-MYH7B antibody [EPR12290] (ab172967)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12290] to MYH7B
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-MYH7B antibody [EPR12290]
See all MYH7B primary antibodies
Description

Rabbit monoclonal [EPR12290] to MYH7B
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Mouse

Human

See all applications and species data
Immunogen

within Human MYH7B. The exact sequence is proprietary.
Database link: A7E2Y1
Positive control
- WB: Human fetal muscle, fetal heart and skeletal muscle lysates. IHC-P: Human skeletal muscle, cardiac muscle, pancreas and heart tissues. ICC/IF: Rat glial tumor cell line.
General notes
The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IHC-P	Human
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR12290
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (ab172967)

Immunohistochemical analysis of paraffin embedded Human heart tissue labeling MYH7B with ab172967 at a 1/250 dilution.
Western blot - Anti-MYH7B antibody [EPR12290] (ab172967)

Anti-MYH7B antibody [EPR12290] (ab172967) at 0.05 µg/ml (Purified) + Human fetal muscle lysate at 20 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 221 kDa
Observed band size: 221 kDa

Blocking and diluting buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (ab172967)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cardiac muscle tissue sections labeling MYH7B with Purified ab172967 at 1:1000 dilution (1.03 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (ab172967)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skeletal muscle tissue sections labeling MYH7B with Purified ab172967 at 1:1000 dilution (1.03 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.
Western blot - Anti-MYH7B antibody [EPR12290] (ab172967)

All lanes : Anti-MYH7B antibody [EPR12290] (ab172967) at 0.1 µg/ml (Purified)

Lane 1 : Mouse heart lysate
Lane 2 : Rat heart lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 221 kDa
Observed band size: 221 kDa

Blocking and diluting buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (ab172967)

ab172967 showing +ve staining in Human normal pancreas tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (ab172967)

Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue labeling MYH7B with ab172967 at a 1/250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (ab172967)

ab172967 showing -ve staining in Human normal liver tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (ab172967)

ab172967 showing -ve staining in Human normal breast tissue.
Western blot - Anti-MYH7B antibody [EPR12290] (ab172967)

All lanes : Anti-MYH7B antibody [EPR12290] (ab172967) at 1/1000 dilution (unpurified)

Lane 1 : Human fetal muscle lysates
Lane 2 : Human fetal heart lysates
Lane 3 : Human skeletal muscle lysates

Lysates/proteins at 10 µg per lane.

Predicted band size: 221 kDa
Anti-MYH7B antibody [EPR12290] (ab172967)

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