Anti-IDOL antibody (ab74562)
Key features and details
- Rabbit polyclonal to IDOL
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IDOL antibody -
Description
Rabbit polyclonal to IDOL -
Host species
Rabbit -
Specificity
ab74562 detects endogenous levels of total IDOL protein. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human IDOL (internal sequence). Immunogen Range:161-210
Database link: Q8WY64
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-IDOL antibody (ab74562)All lanes : Anti-IDOL antibody (ab74562) at 1/500 dilution
Lane 1 : extracts from HeLa cells
Lane 2 : extracts from Jurkat cells
Lane 3 : extracts from Jurkat cells with immunising peptide at 10 µg
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (H+L) HRP at 1/10000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
Additional bands at: 35 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDOL antibody (ab74562)Immunohistochemistry analysis of IDOL in paraffin embedded human brain tissue using 1/50 ab74562.
Left image: untreated sample.
Right image: sample treated with immunising peptide.