Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human HB EGF ELISA Kit (ab100531)

Key features and details

  • Sensitivity: 20 pg/ml
  • Range: 16.38 pg/ml - 4000 pg/ml
  • Sample type: Cell culture supernatant, Plasma
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human HB EGF ELISA Kit

  • Detection method

    Colorimetric

  • Sample type

    Cell culture supernatant, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

  • Range

    16.38 pg/ml - 4000 pg/ml

  • Recovery

    90 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 81.22 73% - 90%
    Plasma 93.73 84% - 103%

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Abcam’s HB EGF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human HB EGF in plasma and cell culture supernatants. (Human HB EGF concentration is pretty low in normal plasma, it may not be detected in this assay).


    This assay employs an antibody specific for Human HB EGF coated on a 96-well plate. Standards and samples are pipetted into the wells and HB EGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human HB EGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HB EGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    We have not been able to detect the endogenous Human HB-EGF in normal serum with ab100531, only in serum spiked with Human HB-EGF.

  • Notes

    Optimization may be required with urine samples

  • Platform

    Microplate

Properties

Properties

Images

  • Typical standard curve
    Typical standard curve

    Representative standard curve using ab100531

  • Typical standard curve
    Typical standard curve

    Representative standard curve using ab100531

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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