Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)
![Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)](https://www.abcam.com/ps/products/108/ab108923/Images/ab108923-121404-anti-myosin-light-chain-3-antibody-epr4160-immunohistochemistry.jpg "Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4160] to Myosin light chain 3
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Myosin light chain 3 antibody [EPR4160]
See all Myosin light chain 3 primary antibodies -
Description
Rabbit monoclonal [EPR4160] to Myosin light chain 3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- human heart muscle lysate, human skeletal muscle lysate; human cardiac muscle tissue, human skeletal muscle tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4160 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)
Immunohistochemical staining of paraffin-embedded human cardiac muscle using ab108923 at a dilution of 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)](https://www.abcam.com/ps/products/108/ab108923/Images/ab108923-121403-anti-myosin-light-chain-3-antibody-epr4160-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)Immunohistochemical staining of paraffin-embedded human skeletal muscle tissue using ab108923 at a diltion of 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)](https://www.abcam.com/ps/products/108/ab108923/Images/ab108923-121402-anti-myosin-light-chain-3-antibody-epr4160-western-blot.jpg)
Western blot - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)All lanes : Anti-Myosin light chain 3 antibody [EPR4160] (ab108923) at 1/1000 dilution
Lane 1 : human heart muscle lysate
Lane 2 : human skeletal muscle lysate
Lysates/proteins at 10 µg/ml per lane.
Predicted band size: 22 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)](https://www.abcam.com/ps/products/108/ab108923/Images/ab108923-121401-anti-myosin-light-chain-3-antibody-epr4160-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)ab108923 showing negative staining in Normal brain tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)](https://www.abcam.com/ps/products/108/ab108923/Images/ab108923-121400-anti-myosin-light-chain-3-antibody-epr4160-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)ab108923 showing negative staining in Normal liver tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)](https://www.abcam.com/ps/products/108/ab108923/Images/ab108923-121399-anti-myosin-light-chain-3-antibody-epr4160-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)ab108923 showing negative staining in Normal tonsil tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)](https://www.abcam.com/ps/products/108/ab108923/Images/ab108923-121398-anti-myosin-light-chain-3-antibody-epr4160-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)ab108923 showing negative staining in Normal breast tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)](https://www.abcam.com/ps/products/108/ab108923/Images/ab108923-8-benefits-of-recombinant-antibodies.png)
Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)
