Anti-Myelin expression factor 2 antibody [EPR10774(B)] (ab170946)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10774(B)] to Myelin expression factor 2
- Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-Myelin expression factor 2 antibody [EPR10774(B)]
See all Myelin expression factor 2 primary antibodies -
Description
Rabbit monoclonal [EPR10774(B)] to Myelin expression factor 2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanIP HumanWB Human -
Immunogen
Synthetic peptide within Human Myelin expression factor 2. The exact sequence is proprietary.
Database link: Q9P2K5 -
Positive control
- Human fetal brain, Jurkat and MCF7 lysates; Human brain and testis tissues; MCF7 and Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10774(B) -
Isotype
IgG -
Research areas
Images
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Western blot analysis of immunoprecipitation pellet from Human fetal brain lysate, labeling Myelin expression factor 2 using ab170946 at a 1/10 dilution.
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All lanes : Anti-Myelin expression factor 2 antibody [EPR10774(B)] (ab170946) at 1/10000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Jurkat cell lysate
Lane 3 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 64 kDa
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Immunohistochemistry analysis of paraffin-embedded Human testis tissue labeling Myelin expression factor 2 with ab170946 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry analysis of Human brain tissue labeling Myelin expression factor 2 with ab170946 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescence analysis of MCF7 cells labeling Myelin expression factor 2 with ab170946 at 1/100 dilution.
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Flow cytometric analysis of permeabilized Jurkat cells labeling Myelin expression factor 2 with ab170946 at 1/10 in red and a rabbit IgG (negative control in green)
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