Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Myelin expression factor 2 antibody [EPR10775(B)] (ab176335)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10775(B)] to Myelin expression factor 2
Suitable for: IHC-P, WB, ICC/IF
Reacts with: Mouse, Human

Product name

Anti-Myelin expression factor 2 antibody [EPR10775(B)]
See all Myelin expression factor 2 primary antibodies
Description

Rabbit monoclonal [EPR10775(B)] to Myelin expression factor 2
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Myelin expression factor 2 aa 550 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q9P2K5
Positive control
- SH-SY5Y, Jurkat, MCF-7, Raw 264.7, fetal brain and fetal liver lysates. Paraffin-embedded Human brain and Human testis tissue. SH-SY5Y cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR10775(B)
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-Myelin expression factor 2 antibody [EPR10775(B)] (ab176335)

Immunofluorescent analysis of SH-SY5Y cells labeling Myelin expression factor 2 with ab176335 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin expression factor 2 antibody [EPR10775(B)] (ab176335)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Myelin expression factor 2 with ab176335 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin expression factor 2 antibody [EPR10775(B)] (ab176335)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Myelin expression factor 2 with ab176335 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-Myelin expression factor 2 antibody [EPR10775(B)] (ab176335)

All lanes : Anti-Myelin expression factor 2 antibody [EPR10775(B)] (ab176335) at 1/1000 dilution

Lane 1 : SH-SY5Y cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : MCF7 lysate
Lane 4 : fetal brain lysate
Lane 5 : fetal liver lysate
Lane 6 : Raw 264.7 lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 64 kDa
Anti-Myelin expression factor 2 antibody [EPR10775(B)] (ab176335)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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