Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Myelin expression factor 2 antibody [EPR10775(B)] (ab176335)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10775(B)] to Myelin expression factor 2
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Myelin expression factor 2 antibody [EPR10775(B)]
    See all Myelin expression factor 2 primary antibodies

  • Description

    Rabbit monoclonal [EPR10775(B)] to Myelin expression factor 2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Myelin expression factor 2 aa 550 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9P2K5

  • Positive control

    • SH-SY5Y, Jurkat, MCF-7, Raw 264.7, fetal brain and fetal liver lysates. Paraffin-embedded Human brain and Human testis tissue. SH-SY5Y cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR10775(B)

  • Isotype

    IgG

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Myelin expression factor 2 antibody [EPR10775(B)] (ab176335)
    Immunocytochemistry/ Immunofluorescence - Anti-Myelin expression factor 2 antibody [EPR10775(B)] (ab176335)

    Immunofluorescent analysis of SH-SY5Y cells labeling Myelin expression factor 2 with ab176335 at 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin expression factor 2 antibody [EPR10775(B)] (ab176335)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin expression factor 2 antibody [EPR10775(B)] (ab176335)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Myelin expression factor 2 with ab176335 at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin expression factor 2 antibody [EPR10775(B)] (ab176335)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin expression factor 2 antibody [EPR10775(B)] (ab176335)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Myelin expression factor 2 with ab176335 at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-Myelin expression factor 2 antibody [EPR10775(B)] (ab176335)
    Western blot - Anti-Myelin expression factor 2 antibody [EPR10775(B)] (ab176335)
    All lanes : Anti-Myelin expression factor 2 antibody [EPR10775(B)] (ab176335) at 1/1000 dilution

    Lane 1 : SH-SY5Y cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : MCF7 lysate
    Lane 4 : fetal brain lysate
    Lane 5 : fetal liver lysate
    Lane 6 : Raw 264.7 lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 64 kDa

  • Anti-Myelin expression factor 2 antibody [EPR10775(B)] (ab176335)
    Anti-Myelin expression factor 2 antibody [EPR10775(B)] (ab176335)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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