Anti-HIR3 antibody (ab61639)
Key features and details
- Rabbit polyclonal to HIR3
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
Overview
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Product name
Anti-HIR3 antibody -
Description
Rabbit polyclonal to HIR3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Saccharomyces cerevisiae
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Immunogen
Synthetic peptide corresponding to Saccharomyces cerevisiae HIR3 aa 1600 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab61638) -
Positive control
- This antibody gave a positive signal in S. cerevisiae Yeast Extract.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab61639 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB Saccharomyces cerevisiaeApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 192 kDa (predicted molecular weight: 192 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 192 kDa (predicted molecular weight: 192 kDa).Target
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Relevance
HIR3 is a member of the HIR complex that also contains HIR1, HIR2 and HPC2. The HIR complex is responsible for the repression of histone gene transcription as well having a role in nucleosome assembly. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 853605 Saccharomyces cerevisiae
- SwissProt: P47171 Saccharomyces cerevisiae
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Alternative names
- Histone transcription regulator 3 antibody
- HPC1 antibody
Images
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Western blot - Anti-HIR3 antibody (ab61639)Anti-HIR3 antibody (ab61639) at 1 µg/ml + S.cerevisiae Yeast Extract at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 192 kDa
Observed band size: 192 kDa
Additional bands at: 30 kDa, 55 kDa, 65 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
Datasheets and documents
References (0)
ab61639 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-HIR3 antibody (ab61639)Anti-HIR3 antibody (ab61639) at 1 µg/ml + S.cerevisiae Yeast Extract at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 192 kDa
Observed band size: 192 kDa
Additional bands at: 30 kDa, 55 kDa, 65 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes