Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MTERF antibody [EPR14202] (ab181836)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14202] to MTERF
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-MTERF antibody [EPR14202]
    See all MTERF primary antibodies

  • Description

    Rabbit monoclonal [EPR14202] to MTERF

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Mouse
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, HepG2 and Jurkat whole cell lysate (ab7899). Human liver and mouse muscle tissue. Jurkat cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR14202

  • Isotype

    IgG

Images

  • Western blot - Anti-MTERF antibody [EPR14202] (ab181836)
    Western blot - Anti-MTERF antibody [EPR14202] (ab181836)
    All lanes : Anti-MTERF antibody [EPR14202] (ab181836) at 1/2000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 46 kDa



    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Western blot - Anti-MTERF antibody [EPR14202] (ab181836)
    Western blot - Anti-MTERF antibody [EPR14202] (ab181836)
    Anti-MTERF antibody [EPR14202] (ab181836) at 1/10000 dilution + HepG2 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 46 kDa



    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTERF antibody [EPR14202] (ab181836)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTERF antibody [EPR14202] (ab181836)

    Immunohistochemical analysis of paraffin embedded Human liver tissue sections labeling MTERF using ab181836 at a 1/250 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTERF antibody [EPR14202] (ab181836)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTERF antibody [EPR14202] (ab181836)

    Immunohistochemical analysis of paraffin embedded mouse muscle tissue sections labeling MTERF using ab181836 at a 1/250 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-MTERF antibody [EPR14202] (ab181836)
    Flow Cytometry - Anti-MTERF antibody [EPR14202] (ab181836)

    Flow Cytometry analysis of Jurkat cells labeling MTERF using ab181836 at a 1/50 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.

  • Anti-MTERF antibody [EPR14202] (ab181836)
    Anti-MTERF antibody [EPR14202] (ab181836)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-MTERF antibody [EPR14202] (ab181836)

© 2021 Astana Biomed Group LLP. All rights reserved.