Anti-BIRC6/APOLLON antibody (ab19609)
Key features and details
- Rabbit polyclonal to BIRC6/APOLLON
- Suitable for: ICC/IF, WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-BIRC6/APOLLON antibody -
Description
Rabbit polyclonal to BIRC6/APOLLON -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Chimpanzee, Rhesus monkey, Orangutan
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Immunogen
Synthetic peptide corresponding to BIRC6/APOLLON (C terminal).
Database link: Q9NR09 -
General notes
This product was previously labelled as BIRC6
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-BIRC6/APOLLON antibody (ab19609)All lanes : Anti-BIRC6/APOLLON antibody (ab19609) at 1/10000 dilution
Lane 1 : HeLa whole cell lysate at 20 µg
Lane 2 : HeLa whole cell lysate at 50 µg
Lane 3 : HeLa S100 extract at 20 µg
Lane 4 : HeLa S100 extract at 50 µg
Predicted band size: 528 kDaDetection of Human BIRC6/APOLLON by western blot.
Lane 1: 20 µg of HeLa whole cell lysate.
Lane 2: 50 µg of HeLa whole cell lysate.
Lane 3: 20 µg of HeLa S100 extract.
Lane 4: 50 µg of HeLa S100 extract.Affinity purified anti-BIRC antibody (ab19609) was used at a 1/10,000 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIRC6/APOLLON antibody (ab19609)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling BIRC6/APOLLON with ab19609 at 1/5000 (0.2µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-BIRC6/APOLLON antibody (ab19609)Detection of human BIRC6/APOLLON by Immunoprecipitation. Affinity purified ab19609 used at 2mcg/mg lysate (whole cell lysate (10mg) from HeLa cells. The immunoprecipitate was resolved by SDS-PAGE and stained with Coomassie Blue. The band that is BIRC6/APOLLON was confirmed by mass spectrometry.
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Immunocytochemistry/ Immunofluorescence - Anti-BIRC6/APOLLON antibody (ab19609)ICC/IF image of ab19609 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19609, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
