Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [7A5A6] (ab233637)

Key features and details

Mouse monoclonal [7A5A6] to Metabotropic Glutamate Receptor 3/MGLUR3
Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
Reacts with: Human
Isotype: IgG2a

Product name

Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [7A5A6]
See all Metabotropic Glutamate Receptor 3/MGLUR3 primary antibodies
Description

Mouse monoclonal [7A5A6] to Metabotropic Glutamate Receptor 3/MGLUR3
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human Metabotropic Glutamate Receptor 3/MGLUR3 aa 433-576 (extracellular).
Database link: Q14832
Positive control
- WB: Metabotropic Glutamate Receptor 3/MGLUR3 (aa 433-576)-hIgGFc transfected HEK-293 cell lysate; Human Metabotropic Glutamate Receptor 3/MGLUR3 (aa 433-576) recombinant protein. ICC/IF: HeLa cells. Flow Cytometry: SH-SY5Y cells. IHC-P: Human cervical cancer tissue.
General notes

This product was previously labelled as Metabotropic Glutamate Receptor 3

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.05% Sodium azide
Constituent: PBS
Concentration information loading...
Purity

Protein G purified
Purification notes

Purified from TCS.
Clonality

Monoclonal
Clone number

7A5A6
Isotype

IgG2a
Light chain type

kappa
Research areas

Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [7A5A6] (ab233637)

All lanes : Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [7A5A6] (ab233637) at 1/500 dilution

Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : Metabotropic Glutamate Receptor 3/MGLUR3 (aa 433-576)-hIgGFc transfected HEK-293 cell lysate

Predicted band size: 99 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [7A5A6] (ab233637)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Metabotropic Glutamate Receptor 3/MGLUR3 (green) using ab233637 at 1/200 dilution in ICC/IF. Secondary antibody: Goat anti-Mouse IgG (H+L) DyLight 488.

Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [7A5A6] (ab233637)

Paraffin-embedded human cervical cancer tissue stained for Metabotropic Glutamate Receptor 3/MGLUR3 using ab233637 at 1/200 dilution in immunohistochemical analysis. DAB staining.
Flow Cytometry - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [7A5A6] (ab233637)

Flow cytometric analysis of SH-SY5Y (human neuroblastoma cell line from bone marrow) cell line labeling Metabotropic Glutamate Receptor 3/MGLUR3 with ab233637 at 1/200 dilution (green) compared with a negative control (red).
Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [7A5A6] (ab233637)

Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [7A5A6] (ab233637) at 1/500 dilution + Human Metabotropic Glutamate Receptor 3/MGLUR3 (aa 433-576) recombinant protein

Predicted band size: 99 kDa

Expected MW is 42.4 kDa.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com