Anti-MEOX 2 antibody (ab174116)
Key features and details
- Rabbit polyclonal to MEOX 2
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MEOX 2 antibody
See all MEOX 2 primary antibodies -
Description
Rabbit polyclonal to MEOX 2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF MouseWB Human
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Immunogen
Synthetic peptide within Human MEOX 2 aa 163-192 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: P50222 -
Positive control
- NIH-3T3 cells; MDA-MB453 cell line lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab174116 is purified through a protein A column, followed by peptide affinity purification. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MEOX 2 antibody (ab174116)Anti-MEOX 2 antibody (ab174116) at 1/100 dilution + MDA-MB453 cell line lysate at 35 µg
Predicted band size: 34 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-MEOX 2 antibody (ab174116)Confocal immunofluorescent analysis of NIH-3T3 cells labeling MEOX 2 with ab174116 at 1/25 dilution. NIH-3T3 cells were fixed with 4% PFA (20 min) and permeabilized with Triton X-100 (0.1%, 10 min) prior to incubation with primary antibody (1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used at 1/400, dilution for 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).
