Anti-MEIS1 antibody (ab19867)
Key features and details
- Rabbit polyclonal to MEIS1
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-MEIS1 antibody
See all MEIS1 primary antibodies -
Description
Rabbit polyclonal to MEIS1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Dog, Zebrafish
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Immunogen
Synthetic peptide corresponding to Human MEIS1 aa 200-300 (internal sequence) conjugated to keyhole limpet haemocyanin.
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Positive control
- This antibody gave a positive signal in the following nuclear cell extract: KG-1. This antibody gave a positive signal in the following FFPE tissue: Human tonsil, and Rat normal brain.
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General notes
For a detailed method for MEIS1 ChIP-seq in RA-treated P19.6 cells, please see the protocol described by Dr Aurelien Serandour at http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM819083.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab19867 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IHC-P (1) Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.WB (1) Use a concentration of 1 µg/ml. Detects a band of approximately 40, 48 kDa (predicted molecular weight: 43, 50 kDa). Abcam recommends using milk as the blocking agent.Notes IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.WB
Use a concentration of 1 µg/ml. Detects a band of approximately 40, 48 kDa (predicted molecular weight: 43, 50 kDa). Abcam recommends using milk as the blocking agent.Target
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Function
Acts as a transcriptional regulator of PAX6. Acts as a transcriptional activator of PF4 in complex with PBX1 or PBX2. Required for hematopoiesis, megakaryocyte lineage development and vascular patterning. May function as a cofactor for HOXA7 and HOXA9 in the induction of myeloid leukemias. -
Tissue specificity
Expressed at low level in normal immunohepatopoietic tissues, including the fetal liver. Expressed in a subset of myeloid leukemia cell lines, with the highest expression seen in those with a megakaryocytic-erythroid phenotype. Also expressed at high levels in the cerebellum. -
Involvement in disease
Defects in MEIS1 could be a cause of susceptibility to restless legs syndrome type 7 (RLS7) [MIM:612853]. Restless legs syndrome (RLS) is a neurologic sleep/wake disorder characterized by uncomfortable and unpleasant sensations in the legs that appear at rest, usually at night, inducing an irresistible desire to move the legs. The disorder results in nocturnal insomnia and chronic sleep deprivation. -
Sequence similarities
Belongs to the TALE/MEIS homeobox family.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4211 Human
- Entrez Gene: 17268 Mouse
- Entrez Gene: 686117 Rat
- Entrez Gene: 170446 Zebrafish
- Omim: 601739 Human
- SwissProt: O00470 Human
- SwissProt: Q60954 Mouse
- Unigene: 526754 Human
see all -
Alternative names
- Homeo box protein Meis1 antibody
- Homeobox protein Meis1 antibody
- Leukemogenic homolog protein antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEIS1 antibody (ab19867)
IHC image of MEIS1 staining in Rat normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab19867, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - Anti-MEIS1 antibody (ab19867)Anti-MEIS1 antibody (ab19867) at 1 µg/ml (blocked with 5% milk) + KG-1 (Human myeloid cell line) Nuclear Lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 43, 50 kDa
Observed band size: 40,48 kDa why is the actual band size different from the predicted?
Additional bands at: 58 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
This antibody was raised against an immunogen that is predicted to recognize isoforms 1 and 2 of MEIS1. The predicted molecular weights of isoforms 1 and 2 are 43 kDa and 50 kDa respectively. Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. -
Western blot - Anti-MEIS1 antibody (ab19867)Anti-MEIS1 antibody (ab19867) at 1 µg/ml + HEK293 Wcl Transiently Overexpressing MEIS1 at 5 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 43, 50 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (33)
ab19867 has been referenced in 33 publications.
- Chiang J et al. The molecular characteristics of low-grade and high-grade areas in desmoplastic infantile astrocytoma/ganglioglioma. Neuropathol Appl Neurobiol 48:e12801 (2022). PubMed: 35191090
- Eagle K et al. Transcriptional Plasticity Drives Leukemia Immune Escape. Blood Cancer Discov 3:394-409 (2022). PubMed: 35709529
- Nagel S & Meyer C Normal and Aberrant TALE-Class Homeobox Gene Activities in Pro-B-Cells and B-Cell Precursor Acute Lymphoblastic Leukemia. Int J Mol Sci 23:N/A (2022). PubMed: 36233173
- Sarkar RK et al. Homeobox transcription factor Meis1 is crucial to Sertoli cell mediated regulation of male fertility. Andrology 9:689-699 (2021). PubMed: 33145986
- Cheng M et al. Transcription Factor ELF1 Activates MEIS1 Transcription and Then Regulates the GFI1/FBW7 Axis to Promote the Development of Glioma. Mol Ther Nucleic Acids 23:418-430 (2021). PubMed: 33473327
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEIS1 antibody (ab19867)
IHC image of MEIS1 staining in Rat normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab19867, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
-
Western blot - Anti-MEIS1 antibody (ab19867)Anti-MEIS1 antibody (ab19867) at 1 µg/ml (blocked with 5% milk) + KG-1 (Human myeloid cell line) Nuclear Lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 43, 50 kDa
Observed band size: 40,48 kDa why is the actual band size different from the predicted?
Additional bands at: 58 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
This antibody was raised against an immunogen that is predicted to recognize isoforms 1 and 2 of MEIS1. The predicted molecular weights of isoforms 1 and 2 are 43 kDa and 50 kDa respectively. Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. -
Western blot - Anti-MEIS1 antibody (ab19867)Anti-MEIS1 antibody (ab19867) at 1 µg/ml + HEK293 Wcl Transiently Overexpressing MEIS1 at 5 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 43, 50 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
