Anti-MEIS1 antibody (ab229962)
Key features and details
- Rabbit polyclonal to MEIS1
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-MEIS1 antibody
See all MEIS1 primary antibodies -
Description
Rabbit polyclonal to MEIS1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human MEIS1 aa 1-90.
Database link: O00470 -
Positive control
- WB: Jurkat, K562 and HeLa whole cell lysate (ab150035); Mouse skeletal muscle lysate. IHC-P: Human prostate cancer tissue. IP: K562 whole cell lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol, PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity > 95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-MEIS1 antibody (ab229962)All lanes : Anti-MEIS1 antibody (ab229962) at 1/2000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : Mouse skeletal muscle lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 43 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEIS1 antibody (ab229962)Paraffin-embedded human prostate cancer tissue stained for MEIS1 using ab229962 at 1/100 dilution in immunohistochemical analysis.
-
Immunoprecipitation - Anti-MEIS1 antibody (ab229962)MEIS1 was immunoprecipitated from 0.5 mg K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate with 4 µg ab229962. Western blot was performed from the immunoprecipitate using ab229962. HRP-conjugated Protein G antibody was used as the secondary antibody at 1/50000 dilution.
Lane 1: 1 µg Rabbit monoclonal IgG instead of ab229962 in K562 whole cell lysate.
Lane 2: ab229962 IP in K562 whole cell lysate.
Lane 3: K562 whole cell lysate 20 µg (Input).
