Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MED12 antibody [BLR084G] (ab275962)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [BLR084G] to MED12
  • Suitable for: WB, IP, IHC-P, ICC
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-MED12 antibody [BLR084G]
    See all MED12 primary antibodies

  • Description

    Rabbit monoclonal [BLR084G] to MED12

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC
    Human
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human MED12 aa 2127-2177 (C terminal).
    Database link: Q93074

  • Positive control

    • WB: HeLa, A549, MCF-7, HepG2, K562, RKO, TCMK-1, and NIH/3T3 cell lysate. IP: HeLa cells. IHC-P: Breast carcinoma, renal carcinoma. ICC: HCT-116 cells.

  • General notes

    This product is sold under License from Bethyl Laboratories, Inc.

Properties

Images

  • Immunoprecipitation - Anti-MED12 antibody [BLR084G] (ab275962)
    Immunoprecipitation - Anti-MED12 antibody [BLR084G] (ab275962)

    Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. 

    ab275962 used for IP at 20 µl/mg lysate.  For blotting immunoprecipitated MED12, ab275962 was used at 1/1000 dilution. Chemiluminescence with an exposure time of 10 seconds.

  • Western blot - Anti-MED12 antibody [BLR084G] (ab275962)
    Western blot - Anti-MED12 antibody [BLR084G] (ab275962)
    All lanes : Anti-MED12 antibody [BLR084G] (ab275962) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : A549 whole cell lysate
    Lane 3 : MCF-7 whole cell lysate
    Lane 4 : HepG2 whole cell lysate
    Lane 5 : K562 whole cell lysate
    Lane 6 : RKO whole cell lysate
    Lane 7 : TCMK-1 whole cell lysate
    Lane 8 : NIH/3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG

    Predicted band size: 243 kDa



    Chemiluminescence with an exposure time of 75 seconds.

    Lower Panel: Rabbit anti-eEF2

  • Immunocytochemistry - Anti-MED12 antibody [BLR084G] (ab275962)
    Immunocytochemistry - Anti-MED12 antibody [BLR084G] (ab275962)

    HCT-116 cells stained for MED12 using ab275962 at 1/500 dilution in immunocytochemistry. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED12 antibody [BLR084G] (ab275962)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED12 antibody [BLR084G] (ab275962)

    Paraffin embedded renal carcinoma tissue stained for MED12 using ab275962 at 1/500 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED12 antibody [BLR084G] (ab275962)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED12 antibody [BLR084G] (ab275962)

    Paraffin embedded breast carcinoma tissue stained for MED12 using ab275962 at 1/500 dilution in immunohistochemical analysis.

  • Anti-MED12 antibody [BLR084G] (ab275962)
    Anti-MED12 antibody [BLR084G] (ab275962)

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