Antibodies, Proteins, Kits and Reagents for Life Science

Anti-MED12 antibody [BLR084G] (ab275962)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR084G] to MED12
Suitable for: WB, IP, IHC-P, ICC
Reacts with: Mouse, Human

Product name

Anti-MED12 antibody [BLR084G]
See all MED12 primary antibodies
Description

Rabbit monoclonal [BLR084G] to MED12
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Human
IP	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide corresponding to Human MED12 aa 2127-2177 (C terminal).
Database link: Q93074
Positive control
- WB: HeLa, A549, MCF-7, HepG2, K562, RKO, TCMK-1, and NIH/3T3 cell lysate. IP: HeLa cells. IHC-P: Breast carcinoma, renal carcinoma. ICC: HCT-116 cells.
General notes

This product is sold under License from Bethyl Laboratories, Inc.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 8.20
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Borate buffered saline
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

BLR084G
Isotype

IgG
Research areas

Immunoprecipitation - Anti-MED12 antibody [BLR084G] (ab275962)

Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.

ab275962 used for IP at 20 µl/mg lysate. For blotting immunoprecipitated MED12, ab275962 was used at 1/1000 dilution. Chemiluminescence with an exposure time of 10 seconds.
Western blot - Anti-MED12 antibody [BLR084G] (ab275962)

All lanes : Anti-MED12 antibody [BLR084G] (ab275962) at 1/1000 dilution

Lane 1 : HeLa whole cell lysate
Lane 2 : A549 whole cell lysate
Lane 3 : MCF-7 whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lane 5 : K562 whole cell lysate
Lane 6 : RKO whole cell lysate
Lane 7 : TCMK-1 whole cell lysate
Lane 8 : NIH/3T3 whole cell lysate

Lysates/proteins at 50 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG

Predicted band size: 243 kDa

Chemiluminescence with an exposure time of 75 seconds.

Lower Panel: Rabbit anti-eEF2
Immunocytochemistry - Anti-MED12 antibody [BLR084G] (ab275962)

HCT-116 cells stained for MED12 using ab275962 at 1/500 dilution in immunocytochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED12 antibody [BLR084G] (ab275962)

Paraffin embedded renal carcinoma tissue stained for MED12 using ab275962 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED12 antibody [BLR084G] (ab275962)

Paraffin embedded breast carcinoma tissue stained for MED12 using ab275962 at 1/500 dilution in immunohistochemical analysis.
Anti-MED12 antibody [BLR084G] (ab275962)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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