Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MCPIP1 antibody [15D11] (ab252879)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [15D11] to MCPIP1
  • Suitable for: WB
  • Reacts with: Mouse

Overview

  • Product name

    Anti-MCPIP1 antibody [15D11]
    See all MCPIP1 primary antibodies

  • Description

    Rat monoclonal [15D11] to MCPIP1

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    ChIP
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: RAW264.7 (treated 4hrs with 100ng/ml LPS) whole cell lysate; F9 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-MCPIP1 antibody [15D11] (ab252879)
    Western blot - Anti-MCPIP1 antibody [15D11] (ab252879)
    All lanes : Anti-MCPIP1 antibody [15D11] (ab252879) at 1/1000 dilution

    Lane 1 : Untreated RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
    Lane 2 : RAW264.7 treated with 100ng/ml LPS (Lipopolysaccharide) for 4 hours, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution (Goat Anti-Rat IgG (H+L), HRP)

    Predicted band size: 65 kDa
    Observed band size: 65 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID: 29263935).

    Exposure time 3 minutes.

  • Western blot - Anti-MCPIP1 antibody [15D11] (ab252879)
    Western blot - Anti-MCPIP1 antibody [15D11] (ab252879)
    Anti-MCPIP1 antibody [15D11] (ab252879) at 1/1000 dilution + F9 (mouse embryonal carcinoma epithelial cell), whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution (Goat Anti-Rat IgG (H+L), HRP)

    Predicted band size: 65 kDa
    Observed band size: 65 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time 20 seconds.

  • Anti-MCPIP1 antibody [15D11] (ab252879)
    Anti-MCPIP1 antibody [15D11] (ab252879)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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