Anti-MCP1 antibody (ab73680)
Key features and details
- Rabbit polyclonal to MCP1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MCP1 antibody
See all MCP1 primary antibodies -
Description
Rabbit polyclonal to MCP1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human MCP1 aa 50 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab73679) -
Positive control
- This antibody gave a positive signal in the following Human Whole Cell Lysates: HCT 116, HeLa
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab73680 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications ChimpanzeeRhesus monkeyOrangutanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 11 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 11 kDa).Target
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Function
Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsProcessing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 6347 Human
- Omim: 158105 Human
- SwissProt: P13500 Human
- Unigene: 303649 Human
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Alternative names
- C-C motif chemokine 2 antibody
- CCL2 antibody
- CCL2_HUMAN antibody
see all
Images
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Western blot - Anti-MCP1 antibody (ab73680)All lanes : Anti-MCP1 antibody (ab73680) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 11 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Additional bands at: 125 kDa, 50 kDa (possible glycosylated form), 80 kDa. We are unsure as to the identity of these extra bands.
The expression profile observed is consistent with what has been described in the literature (e.g. PMID 12957789). We believe the 50kDa band is a glycosylated form of Human MCP1.
Datasheets and documents
References (4)
ab73680 has been referenced in 4 publications.
- Tan X et al. Induction of SPARC on Oxidative Stress, Inflammatory Phenotype Transformation, and Apoptosis of Human Brain Smooth Muscle Cells Via TGF-ß1-NOX4 Pathway. J Mol Neurosci N/A:N/A (2020). PubMed: 32495004
- Kobayashi K et al. Role of monocyte chemoattractant protein-1 in liver fibrosis with transient myeloproliferative disorder in down syndrome. Hepatol Commun 2:230-236 (2018). PubMed: 29507898
- Zabini D et al. Comprehensive analysis of inflammatory markers in chronic thromboembolic pulmonary hypertension patients. Eur Respir J 44:951-62 (2014). IHC . PubMed: 25034560
- Tjomsland V et al. The desmoplastic stroma plays an essential role in the accumulation and modulation of infiltrated immune cells in pancreatic adenocarcinoma. Clin Dev Immunol 2011:212810 (2011). IHC-P ; Human . PubMed: 22190968
Images
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Western blot - Anti-MCP1 antibody (ab73680)All lanes : Anti-MCP1 antibody (ab73680) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 11 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Additional bands at: 125 kDa, 50 kDa (possible glycosylated form), 80 kDa. We are unsure as to the identity of these extra bands.
The expression profile observed is consistent with what has been described in the literature (e.g. PMID 12957789). We believe the 50kDa band is a glycosylated form of Human MCP1.
