Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CXCL9 antibody [EPR23999-5] - BSA and Azide free (ab277639)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23999-5] to CXCL9 - BSA and Azide free
  • Suitable for: WB, IP, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CXCL9 antibody [EPR23999-5] - BSA and Azide free
    See all CXCL9 primary antibodies

  • Description

    Rabbit monoclonal [EPR23999-5] to CXCL9 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC or IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: THP-1 (treated with 200ng/ml IFN-? and 50 ng/ml LPS(Lipopolysaccharide) for 24 hours) whole cell lysate; His-tagged human purified recombinant full-length protein CXCL9. Flow cyt: THP-1 (treated with 100ng/ml IFN-gamma and 1ug/ml LPS(Lipopolysaccharide) for 48 hours) cells. IP: THP-1 (treated with 100ng/ml IFN-gamma and 1ug/ml LPS(Lipopolysaccharide) for 48 hours) whole cell lysate.

  • General notes

    ab277639 is the carrier-free version of ab263442. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab277639 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23999-5

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-CXCL9 antibody [EPR23999-5] - BSA and Azide free (ab277639)
    Western blot - Anti-CXCL9 antibody [EPR23999-5] - BSA and Azide free (ab277639)
    All lanes : Anti-CXCL9 antibody [EPR23999-5] (ab263442) at 1/1000 dilution

    Lane 1 : Untreated THP-1 (human monocytic leukemia monocyte), whole cell lysate
    Lane 2 : THP-1 treated with /ml IFN-gamma and 50 ng/ml LPS(Lipopolysaccharide) for 24 hours, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

    Predicted band size: 14 kDa
    Observed band size: 13,14 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab263442, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile is consistent with what has been described in the literature (PMID: 15154616, 22973274).

    Exposure time: 3 minutes.

  • Flow Cytometry - Anti-CXCL9 antibody [EPR23999-5] - BSA and Azide free (ab277639)
    Flow Cytometry - Anti-CXCL9 antibody [EPR23999-5] - BSA and Azide free (ab277639)

    This data was developed using ab263442, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized THP-1 (human monocytic leukemia monocyte) treated with 100ng/ml IFN-γand 1ug/ml LPS (Lipopolysaccharide) for 48 hours (Red)/ Untreated control (Green) cells labelling CXCL9 with ab263442 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730)  isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-CXCL9 antibody [EPR23999-5] - BSA and Azide free (ab277639)
    Immunoprecipitation - Anti-CXCL9 antibody [EPR23999-5] - BSA and Azide free (ab277639)

    This data was developed using ab263442, the same antibody clone in a different buffer formulation.

    CXCL9 was immunoprecipitated from 0.35 mg THP-1(human monocytic leukemia monocyte) (treated with 100ng/ml IFN-γ and 1ug/ml LPS (Lipopolysaccharide) for 48 hours) whole cell lysate with ab263442 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab263442 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: THP-1 treated with 100ng/ml IFN-γ and 1ug/ml LPS (Lipopolysaccharide) for 48 hours, whole cell lysate 10 ug

    Lane 2: ab263442 IP in THP-1 treated with 100ng/ml IFN-γ and 1ug/ml LPS(Lipopolysaccharide) for 48 hours whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab263442 in THP-1 treated with 100ng/ml IFN-γ and 1ug/ml LPS(Lipopolysaccharide) for 48 hours whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Western blot - Anti-CXCL9 antibody [EPR23999-5] - BSA and Azide free (ab277639)
    Western blot - Anti-CXCL9 antibody [EPR23999-5] - BSA and Azide free (ab277639)
    Anti-CXCL9 antibody [EPR23999-5] (ab263442) at 1/1000 dilution + His-tagged human purified recombinant full-length protein CXCL9, 10 ng

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

    Predicted band size: 14 kDa
    Observed band size: 13,14 kDa why is the actual band size different from the predicted?



    This data was developed using ab263442, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile is consistent with what has been described in the literature (PMID: 15154616, 22973274).

    Exposure time: 5.5 seconds.

  • Anti-CXCL9 antibody [EPR23999-5] - BSA and Azide free (ab277639)
    Anti-CXCL9 antibody [EPR23999-5] - BSA and Azide free (ab277639)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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