Anti-MCP1 antibody [EP1361] (ab151538)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1361] to MCP1
- Suitable for: WB
- Reacts with: Human, Recombinant fragment
Overview
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Product name
Anti-MCP1 antibody [EP1361]
See all MCP1 primary antibodies -
Description
Rabbit monoclonal [EP1361] to MCP1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB HumanRecombinant fragment -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human MCP1 aa 50 to the C-terminus.
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Positive control
- Purchase matching WB positive control:Recombinant human MCP1 protein
- WB: Human fetal brain tissue lysate and human MCP1 recombinant protein.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1361 -
Isotype
IgG -
Research areas
Images
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Anti-MCP1 antibody [EP1361] (ab151538) at 1/5000 dilution (purified) + MCP-1 recombinant protein at 0.01 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 11 kDa
Observed band size: 11 kDaBlocking and diluting buffer: 5% NFDM/TBST.
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Anti-MCP1 antibody [EP1361] (ab151538) at 1/5000 dilution (purified) + Human fetal brain tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 11 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST.
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All lanes : Anti-MCP1 antibody [EP1361] (ab151538) at 1/1000 dilution (unpurified)
Lane 1 : Human fetal brain tissue lysate
Lane 2 : Recombinant human MCP1 (76aa, 8.6kDa)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 11 kDa
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