Anti-MCP1 antibody (ab9669)
Key features and details
- Rabbit polyclonal to MCP1
- Suitable for: WB, Sandwich ELISA
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-MCP1 antibody
See all MCP1 primary antibodies -
Description
Rabbit polyclonal to MCP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Sandwich ELISAmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant fragment corresponding to Human MCP1.
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Positive control
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General notes
Although some customers have been successful in IHC we no longer batch test in this application. For and IHC validated antibody please see ab73680
Properties
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Form
Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Light chain type
unknown -
Research areas
Images
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Western blot - Anti-MCP1 antibody (ab9669)
HL-60 cells were incubated at 37°C for 24hrs with vehicle control (0 µM) and varied concentrations of 2-Arachidonylglycerol (ab120098). Increased expression of MCP1 in HL-60 cells correlates with an increase in 2-Arachidonylglycerol concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab9669 at 1 µg/ml and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
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Sandwich ELISA - Anti-MCP1 antibody (ab9669)Standard curve for MCP1 (Analyte: ab73866); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal to MCP1 (ab9858) at 1µg/ml and Detector Antibody Rabbit polyclonal to MCP1 (ab9669) at 0.5µg/ml. -
Western blot - Anti-MCP1 antibody (ab9669)All lanes : Anti-MCP1 antibody (ab9669) at 0.2 µg/ml
Lane 1 :Recombinant human MCP1 protein (ab73866) at 0.01 µg
Lane 2 :Recombinant human MCP1 protein (ab73866) at 0.001 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 1 minute -
Western blot - Anti-MCP1 antibody (ab9669) Image courtesy of Dr Laura Toma by Abreview.Anti-MCP1 antibody (ab9669) at 1/4000 dilution + lysate prepared from human smooth muscle treated with 100µg/ml glycated LDL at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/20000 dilution
Developed using the ECL technique.
Observed band size: 12-14 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
