Anti-FOXP4 antibody (ab17726)
Key features and details
- Rabbit polyclonal to FOXP4
- Suitable for: IHC-FoFr, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-FOXP4 antibody
See all FOXP4 primary antibodies -
Description
Rabbit polyclonal to FOXP4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-FoFr MouseWB Human
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human FOXP4.
Read Abcam's proprietary immunogen policy (Peptide available as ab20334.) -
Positive control
- Human FOXP4 Partial Recombinant Protein (tagged) Mouse stomach tissue lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab17726 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species IHC-FoFr MouseWB HumanApplication Abreviews Notes IHC-FoFr WB (1) Notes Application notesIHC-F: 1/100 - 1/300.
WB: Use at a concentration of 1 µg/ml (Human FOXP4 Partial Recombinant Protein). Used at a dilution of 1/1000 for 12 hrs incubation on human cells (see Abreview for further details).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.Target
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Function
Transcriptional repressor that represses lung-specific expression. -
Sequence similarities
Contains 1 C2H2-type zinc finger.
Contains 1 fork-head DNA-binding domain. -
Domain
The leucine-zipper is required for dimerization and transcriptional repression. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 116113 Human
- Entrez Gene: 74123 Mouse
- Omim: 608924 Human
- SwissProt: Q5W098 Human
- SwissProt: Q8IVH2 Human
- SwissProt: Q80V92 Mouse
- SwissProt: Q8CG10 Mouse
- SwissProt: Q8CIS1 Mouse
see all -
Alternative names
- 1200010K03Rik antibody
- 2310007G05Rik antibody
- FKHLA antibody
see all
Images
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-FOXP4 antibody (ab17726)This image is courtesy of Shunyou Wang, Ph.D.ab17726 was tested in IHC of formalin fixed sections from mouse (over two weeks of age) stomach. Antigen retrieval was in citrate buffer and blocking was in 1% BSA. The primary antibody was incubated at a dilution of 1/100 overnight at 4 degrees celsius. The secondary antibody was a biotin conjugated anti-rabbit IgG.
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Western blot - Anti-FOXP4 antibody (ab17726)This image is courtesy of an anonymous AbreviewAll lanes : Anti-FOXP4 antibody (ab17726) at 1/1000 dilution
Lane 1 : 50ug Lung epithelial cell line, non-transfected.
Lane 2 : 50ug Lung epithelial cell line, transfected with FOXP4.
Secondary
All lanes : HRP cpnjugated goat anti-rabbit
Performed under reducing conditions.
Predicted band size: 11 kDa
Exposure time: 1 second
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Western blot - Anti-FOXP4 antibody (ab17726)Anti-FOXP4 antibody (ab17726) at 1 µg/ml + Human FOXP4 Partial Recombinant Protein (Tagged) at 0.1 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 11 kDa
Observed band size: 11 kDa
Protocols
Datasheets and documents
References (4)
ab17726 has been referenced in 4 publications.
- Li Y et al. MicroRNA-4651 represses hepatocellular carcinoma cell growth and facilitates apoptosis via targeting FOXP4. Biosci Rep 40:N/A (2020). PubMed: 32436934
- Xu Y et al. MicroRNA-299-3p/FOXP4 Axis Regulates the Proliferation and Migration of Oral Squamous Cell Carcinoma. Technol Cancer Res Treat 18:1533033819874803 (2019). PubMed: 31500519
- Yin Z et al. Up-regulation of microRNA-491-5p suppresses cell proliferation and promotes apoptosis by targeting FOXP4 in human osteosarcoma. Cell Prolif 50:N/A (2017). PubMed: 27704627
- Spiteri E et al. Identification of the transcriptional targets of FOXP2, a gene linked to speech and language, in developing human brain. Am J Hum Genet 81:1144-57 (2007). PubMed: 17999357
Images
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-FOXP4 antibody (ab17726) This image is courtesy of Shunyou Wang, Ph.D.ab17726 was tested in IHC of formalin fixed sections from mouse (over two weeks of age) stomach. Antigen retrieval was in citrate buffer and blocking was in 1% BSA. The primary antibody was incubated at a dilution of 1/100 overnight at 4 degrees celsius. The secondary antibody was a biotin conjugated anti-rabbit IgG.
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Western blot - Anti-FOXP4 antibody (ab17726) This image is courtesy of an anonymous AbreviewAll lanes : Anti-FOXP4 antibody (ab17726) at 1/1000 dilution
Lane 1 : 50ug Lung epithelial cell line, non-transfected.
Lane 2 : 50ug Lung epithelial cell line, transfected with FOXP4.
Secondary
All lanes : HRP cpnjugated goat anti-rabbit
Performed under reducing conditions.
Predicted band size: 11 kDa
Exposure time: 1 second
-
Western blot - Anti-FOXP4 antibody (ab17726)Anti-FOXP4 antibody (ab17726) at 1 µg/ml + Human FOXP4 Partial Recombinant Protein (Tagged) at 0.1 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 11 kDa
Observed band size: 11 kDa