Anti-Mannosidase II/MAN2A1 antibody (ab12277)
Key features and details
- Rabbit polyclonal to Mannosidase II/MAN2A1
- Suitable for: ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-Mannosidase II/MAN2A1 antibody
See all Mannosidase II/MAN2A1 primary antibodies -
Description
Rabbit polyclonal to Mannosidase II/MAN2A1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanAfrican green monkey
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Immunogen
Synthetic peptide corresponding to Human Mannosidase II/MAN2A1 (internal sequence) conjugated to keyhole limpet haemocyanin.
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Positive control
- ICC: COS7 cells
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General notes
This product was previously labelled as Mannosidase II
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Mannosidase II/MAN2A1 antibody (ab12277)COS7 cells were fixed with cold methanol for 10 minutes followed by incubation with ab12277 (1/200) in 5% normal goat serum. FITC-conjugated goat anti-rabbit was used to visualise bound rabbit antibodies. Nuclei were stained with DAPI.
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Immunocytochemistry/ Immunofluorescence - Anti-Mannosidase II/MAN2A1 antibody (ab12277)ICC/IF image of ab12277 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12277, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
