Anti-M6PR (cation independent) antibody (ab12894)
Key features and details
- Rabbit polyclonal to M6PR (cation independent)
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-M6PR (cation independent) antibody
See all M6PR (cation independent) primary antibodies -
Description
Rabbit polyclonal to M6PR (cation independent) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Immunogen
Synthetic peptide corresponding to Human M6PR (cation independent) aa 700-800 (internal sequence) conjugated to keyhole limpet haemocyanin.
(Peptide available asab13210)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab12894 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanAll applications MouseMonkeyAfrican green monkeyApplication Abreviews Notes ICC/IF (3) Use a concentration of 4 µg/ml. This antibody worked well in IF in HeLa cells but not in SK-N-SH cells.Notes ICC/IF
Use a concentration of 4 µg/ml. This antibody worked well in IF in HeLa cells but not in SK-N-SH cells.Target
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Function
Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4. -
Sequence similarities
Belongs to the MRL1/IGF2R family.
Contains 1 fibronectin type-II domain. -
Domain
Contains 15 repeating units of approximately 147 AA harboring four disulfide bonds each. The most highly conserved region within the repeat consists of a stretch of 13 AA that contains cysteines at both ends. -
Cellular localization
Lysosome membrane. Colocalized with DPP4 in internalized cytoplasmic vesicles adjacent to the cell surface. - Information by UniProt
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Database links
- Entrez Gene: 3482 Human
- Entrez Gene: 16004 Mouse
- Omim: 147280 Human
- SwissProt: P11717 Human
- SwissProt: Q07113 Mouse
- Unigene: 487062 Human
- Unigene: 673278 Human
- Unigene: 26553 Mouse
see all -
Alternative names
- 300 kDa mannose 6 phosphate receptor antibody
- 300 kDa mannose 6-phosphate receptor antibody
- Cation independent mannose 6 phosphate receptor antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody (ab12894)This image was submitted as part of a review by Kirk McManus, University of British Columbia
HeLa cells labelled with anti-mannose-6-phosphate receptor (ab12894). ab12894 was used at a working concentration of 4µg/ml. The arrows indicate the perinuclear location of the mannose-6-phosphate receptor in the late endosomes. In the final panel DAPI is pseudo-coloured red, while the mannose-6-phosphate receptor is in green.
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Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody (ab12894)This image is courtesy of an anonymous Abreviewab12894 at 1/100 staining human MCF-7 cells by ICC/IF. The cells were methanol fixed and blocked with BSA prior to incubation with the antibody for 1 hour. A goat anti-rabbit FITC was used as the secondary.
Protocols
References (5)
ab12894 has been referenced in 5 publications.
- Fong LK et al. Full-length amyloid precursor protein regulates lipoprotein metabolism and amyloid-ß clearance in human astrocytes. J Biol Chem 293:11341-11357 (2018). PubMed: 29858247
- Liu Y et al. Endocytic processing of adeno-associated virus type 8 vectors for transduction of target cells. Gene Ther : (2012). Human . PubMed: 22622241
- Shen Y et al. Mutations in PNKD causing paroxysmal dyskinesia alters protein cleavage and stability. Hum Mol Genet 20:2322-32 (2011). ICC/IF ; Human . PubMed: 21487022
- Nishitsuji K et al. The E693Delta mutation in amyloid precursor protein increases intracellular accumulation of amyloid beta oligomers and causes endoplasmic reticulum stress-induced apoptosis in cultured cells. Am J Pathol 174:957-69 (2009). ICC/IF ; African green monkey . PubMed: 19164507
- Clarke JH et al. Distribution and neuronal expression of phosphatidylinositol phosphate kinase IIgamma in the mouse brain. J Comp Neurol 517:296-312 (2009). PubMed: 19757494
Images
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Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody (ab12894) This image was submitted as part of a review by Kirk McManus, University of British Columbia
HeLa cells labelled with anti-mannose-6-phosphate receptor (ab12894). ab12894 was used at a working concentration of 4µg/ml. The arrows indicate the perinuclear location of the mannose-6-phosphate receptor in the late endosomes. In the final panel DAPI is pseudo-coloured red, while the mannose-6-phosphate receptor is in green.
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Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody (ab12894) This image is courtesy of an anonymous Abreviewab12894 at 1/100 staining human MCF-7 cells by ICC/IF. The cells were methanol fixed and blocked with BSA prior to incubation with the antibody for 1 hour. A goat anti-rabbit FITC was used as the secondary.
