Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Kininogen 1 antibody [EPR6097] (ab124737)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6097] to Kininogen 1
  • Suitable for: ELISA, WB, IP, IHC-P
  • Reacts with: Human, Recombinant fragment

Overview

  • Product name

    Anti-Kininogen 1 antibody [EPR6097]
    See all Kininogen 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR6097] to Kininogen 1

  • Host species

    Rabbit

  • Specificity

    This antibody detects three isoforms of Kininogen 1: full length (120 kDa), light chain (56 kDa), and cleavage form (45 kDa).

  • Tested Applications & Species

    Application Species
    ELISA
    Recombinant fragment
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Kininogen 1 aa 600 to the C-terminus. The exact sequence is proprietary.

  • Positive control

    • Human plasma, platelet and ovarian cancer lysates; Human kidney tissue, Human ovarian adenocarcinoma tissue.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 2.70 x 10 -12 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR6097

  • Isotype

    IgG

Images

  • Western blot - Anti-Kininogen 1 antibody [EPR6097] (ab124737)
    Western blot - Anti-Kininogen 1 antibody [EPR6097] (ab124737)
    All lanes : Anti-Kininogen 1 antibody [EPR6097] (ab124737) at 1/10000 dilution

    Lane 1 : Human plasma lysate
    Lane 2 : Human platelet lysate
    Lane 3 : Human ovarian cancer lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 48, 72 kDa



    ab124737 is detecting three isoforms of Kininogen 1: full length (120 kDa), light chain (56 kDa), and cleavage form (45 kDa).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kininogen 1 antibody [EPR6097] (ab124737)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kininogen 1 antibody [EPR6097] (ab124737)

    ab124737 at 1/100 dilution staining Kininogen 1 in paraffin-embedded Human kidney tissues by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kininogen 1 antibody [EPR6097] (ab124737)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kininogen 1 antibody [EPR6097] (ab124737)

    ab124737 at 1/100 dilution staining Kininogen 1 in paraffin-embedded Human ovarian adenocarcinoma tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ELISA - Anti-Kininogen 1 antibody [EPR6097] (ab124737)
    ELISA - Anti-Kininogen 1 antibody [EPR6097] (ab124737)
    ELISA analysis of Human Kininogen-1 recombinant protein at 1000 ng/mL with ab124737. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
  • OI-RD Scanning - Anti-Kininogen 1 antibody [EPR6097] (ab124737)
    OI-RD Scanning - Anti-Kininogen 1 antibody [EPR6097] (ab124737)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Kininogen 1 antibody [EPR6097] (ab124737)
    Anti-Kininogen 1 antibody [EPR6097] (ab124737)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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