Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MK-3 antibody [EPR13969] (ab183040)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13969] to MK-3
  • Suitable for: WB, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-MK-3 antibody [EPR13969]
    See all MK-3 primary antibodies

  • Description

    Rabbit monoclonal [EPR13969] to MK-3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa and Jurkat cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13969

  • Isotype

    IgG

  • Research areas

Images

  • Immunoprecipitation - Anti-MK-3 antibody [EPR13969] (ab183040)
    Immunoprecipitation - Anti-MK-3 antibody [EPR13969] (ab183040)

    Immunoprecipitation analysis of Jurkat cells labeling MK-3 using ab183040 at 1/50 dilution. A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody.

  • Flow Cytometry - Anti-MK-3 antibody [EPR13969] (ab183040)
    Flow Cytometry - Anti-MK-3 antibody [EPR13969] (ab183040)

    Flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling MK-3 with ab183040 at 1/100 followed by Goat anti rabbit IgG (FITC) at 1/150 (pink).  Rabbit monoclonal IgG was used as Isotype control (green).

  • Western blot - Anti-MK-3 antibody [EPR13969] (ab183040)
    Western blot - Anti-MK-3 antibody [EPR13969] (ab183040)
    Anti-MK-3 antibody [EPR13969] (ab183040) at 1/5000 dilution + Jurkat cell line lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 43 kDa

  • Western blot - Anti-MK-3 antibody [EPR13969] (ab183040)
    Western blot - Anti-MK-3 antibody [EPR13969] (ab183040)
    Anti-MK-3 antibody [EPR13969] (ab183040) at 1/20000 dilution + HeLa cell line lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 43 kDa

  • Anti-MK-3 antibody [EPR13969] (ab183040)
    Anti-MK-3 antibody [EPR13969] (ab183040)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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