Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Livin antibody [OTI1B10] (ab236500)

Key features and details

Mouse monoclonal [OTI1B10] to Livin
Suitable for: WB, ICC/IF, Flow Cyt
Reacts with: Human, Recombinant fragment
Isotype: IgG1

Product name

Anti-Livin antibody [OTI1B10]
See all Livin primary antibodies
Description

Mouse monoclonal [OTI1B10] to Livin
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human Recombinant fragment
WB	Human

See all applications and species data

Immunogen

Recombinant full length protein corresponding to Human Livin aa 1 to the C-terminus. Produced in HEK293T cells (NP_071444).
Database link: Q96CA5-2

Run BLAST with Run BLAST with
Positive control
- WB: pCMV6-ENTRY Livin cDNA transfected HEK-293T cell lysate. Flow Cyt: Jurkat and HeLa cells. ICC/IF: COS-7 cells transiently transfected by pCMV6-ENTRY Livin; HeLa cells.
General notes

Clone OTI1B10 (formerly 1B10).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purity

Affinity purified
Purification notes

Purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

OTI1B10
Isotype

IgG1
Research areas

Western blot - Anti-Livin antibody [OTI1B10] (ab236500)

All lanes : Anti-Livin antibody [OTI1B10] (ab236500) at 1/2000 dilution

Lane 1 : pCMV6-ENTRY control cDNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : pCMV6-ENTRY Livin cDNA transfected HEK-293T cell lysate

Lysates/proteins at 5 µg per lane.

Predicted band size: 33 kDa
Flow Cytometry - Anti-Livin antibody [OTI1B10] (ab236500)

Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cell line labeling Livin with ab236500 at 1/100 dilution (red) compared to a nonspecific negative control antibody (blue).
Flow Cytometry - Anti-Livin antibody [OTI1B10] (ab236500)

Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling Livin with ab236500 at 1/100 dilution (red) compared to a nonspecific negative control antibody (blue).
Immunocytochemistry/ Immunofluorescence - Anti-Livin antibody [OTI1B10] (ab236500)

COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY Livin stained for Livin (green) using ab236500 at 1/100 dilution in ICC/IF.
Immunocytochemistry/ Immunofluorescence - Anti-Livin antibody [OTI1B10] (ab236500)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Livin (green) using ab236500 at 1/100 dilution in ICC/IF.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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