Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Livin antibody [OTI1B10] (ab236500)

Key features and details

  • Mouse monoclonal [OTI1B10] to Livin
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG1

Overview

  • Product name

    Anti-Livin antibody [OTI1B10]
    See all Livin primary antibodies

  • Description

    Mouse monoclonal [OTI1B10] to Livin

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    Recombinant fragment
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein corresponding to Human Livin aa 1 to the C-terminus. Produced in HEK293T cells (NP_071444).
    Database link: Q96CA5-2

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: pCMV6-ENTRY Livin cDNA transfected HEK-293T cell lysate. Flow Cyt: Jurkat and HeLa cells. ICC/IF: COS-7 cells transiently transfected by pCMV6-ENTRY Livin; HeLa cells.

  • General notes

    Clone OTI1B10 (formerly 1B10).

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-Livin antibody [OTI1B10] (ab236500)
    Western blot - Anti-Livin antibody [OTI1B10] (ab236500)
    All lanes : Anti-Livin antibody [OTI1B10] (ab236500) at 1/2000 dilution

    Lane 1 : pCMV6-ENTRY control cDNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 2 : pCMV6-ENTRY Livin cDNA transfected HEK-293T cell lysate

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 33 kDa

  • Flow Cytometry - Anti-Livin antibody [OTI1B10] (ab236500)
    Flow Cytometry - Anti-Livin antibody [OTI1B10] (ab236500)

    Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cell line labeling Livin with ab236500 at 1/100 dilution (red) compared to a nonspecific negative control antibody (blue).

  • Flow Cytometry - Anti-Livin antibody [OTI1B10] (ab236500)
    Flow Cytometry - Anti-Livin antibody [OTI1B10] (ab236500)

    Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling Livin with ab236500 at 1/100 dilution (red) compared to a nonspecific negative control antibody (blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-Livin antibody [OTI1B10] (ab236500)
    Immunocytochemistry/ Immunofluorescence - Anti-Livin antibody [OTI1B10] (ab236500)

    COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY Livin stained for Livin (green) using ab236500 at 1/100 dilution in ICC/IF.

  • Immunocytochemistry/ Immunofluorescence - Anti-Livin antibody [OTI1B10] (ab236500)
    Immunocytochemistry/ Immunofluorescence - Anti-Livin antibody [OTI1B10] (ab236500)

    HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Livin (green) using ab236500 at 1/100 dilution in ICC/IF.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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