Anti-LIM2 antibody [EPR16947] - BSA and Azide free (ab251257)
![Anti-LIM2 antibody [EPR16947] - BSA and Azide free (ab251257)](https://www.abcam.com/ps/products/251/ab251257/Images/ab251257-408144-anti-lim2-antibody-epr16947-bsa-and-azide-free-western-blot-mouse.jpg "Anti-LIM2 antibody [EPR16947] - BSA and Azide free (ab251257)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16947] to LIM2 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-LIM2 antibody [EPR16947] - BSA and Azide free
See all LIM2 primary antibodies -
Description
Rabbit monoclonal [EPR16947] to LIM2 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251257 is the carrier-free version of ab198502. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251257 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR16947 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-LIM2 antibody [EPR16947] - BSA and Azide free (ab251257)All lanes : Anti-LIM2 antibody [EPR16947] (ab198502) at 1/50000 dilution
Lane 1 : Mouse eyeball tissue lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Mouse heart tissue lysate
Lane 4 : Mouse kidney tissue lysate
Lane 5 : Mouse spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 20 kDa
Observed band size: 20 kDa
Exposure time: 30 secondsThis data was developed using ab198502, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-LIM2 antibody [EPR16947] - BSA and Azide free (ab251257)](https://www.abcam.com/ps/products/251/ab251257/Images/ab251257-405799-anti-lim2-antibody-epr16947-bsa-and-azide-free-western-blot-rat.jpg)
Western blot - Anti-LIM2 antibody [EPR16947] - BSA and Azide free (ab251257)All lanes : Anti-LIM2 antibody [EPR16947] (ab198502) at 1/5000 dilution
Lane 1 : Rat eyeball tissue lysate
Lane 2 : Rat brain tissue lysate
Lane 3 : Rat heart tissue lysate
Lane 4 : Rat kidney tissue lysate
Lane 5 : Rat spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 20 kDa
Observed band size: 20 kDa
Exposure time: 3 minutesThis data was developed using ab198502, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Anti-LIM2 antibody [EPR16947] - BSA and Azide free (ab251257)](https://www.abcam.com/ps/products/251/ab251257/Images/ab251257-3-benefits-of-recombinant-antibodies.png)
Anti-LIM2 antibody [EPR16947] - BSA and Azide free (ab251257)