Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Rabbit polyclonal to LIGHT/TNFSF14
Suitable for: WB, Sandwich ELISA
Reacts with: Recombinant fragment
Isotype: IgG

Product name

Anti-LIGHT/TNFSF14 antibody
See all LIGHT/TNFSF14 primary antibodies
Description

Rabbit polyclonal to LIGHT/TNFSF14
Host species

Rabbit
Tested Applications & Species

Application Species
sELISA
Recombinant fragment

WB
Recombinant fragment

See all applications and species data
Immunogen

Recombinant fragment corresponding to Mouse LIGHT/TNFSF14 aa 50 to the C-terminus. (E.coli derived).
Database link: Q9QYH9

Run BLAST with Run BLAST with
Positive control
- WB: Recombinant mouse LIGHT/TNFSF14 protein.
General notes

This product was previously labelled as LIGHT

Application	Species
sELISA	Recombinant fragment
WB	Recombinant fragment

Form

Lyophilized:For lot specific reconstitution information please contact our Scientific Support Team.
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Constituent: PBS

Sterile filtered
Concentration information loading...
Purity

Immunogen affinity purified
Purification notes

ab233502 was purified by affinity chromatography employing an immobilized Murine LIGHT/TNFSF14 matrix.
Clonality

Polyclonal
Isotype

IgG
Research areas

Western blot - Anti-LIGHT/TNFSF14 antibody (ab233502)

All lanes : Anti-LIGHT/TNFSF14 antibody (ab233502) at 0.2 µg/ml

Lane 1 : Recombinant mouse LIGHT/TNFSF14 protein, 250 ng
Lane 2 : Recombinant mouse LIGHT/TNFSF14 protein, 125 ng
Lane 3 : Recombinant mouse LIGHT/TNFSF14 protein, 62.5 ng
Lane 4 : Recombinant mouse LIGHT/TNFSF14 protein, 31.25 ng
Lane 5 : Recombinant mouse LIGHT/TNFSF14 protein, 15.625 ng
Lane 6 : Recombinant mouse LIGHT/TNFSF14 protein, 7.8 ng
Lane 7 : Recombinant mouse LIGHT/TNFSF14 protein, 3.9 ng
Lane 8 : Recombinant mouse LIGHT/TNFSF14 protein, 1.95 ng
Lane 9 : Recombinant mouse LIGHT/TNFSF14 protein, 0.975 ng
Lane 10 : Recombinant mouse LIGHT/TNFSF14 protein, 0.49 ng
Lane 11 : Recombinant mouse LIGHT/TNFSF14 protein, 0.24 ng

Predicted band size: 26 kDa

Reducing conditions.
Western blot - Anti-LIGHT/TNFSF14 antibody (ab233502)

All lanes : Anti-LIGHT/TNFSF14 antibody (ab233502) at 0.1 µg/ml

Lane 1 : Recombinant mouse LIGHT/TNFSF14 protein, 250 ng
Lane 2 : Recombinant mouse LIGHT/TNFSF14 protein, 125 ng
Lane 3 : Recombinant mouse LIGHT/TNFSF14 protein, 62.5 ng
Lane 4 : Recombinant mouse LIGHT/TNFSF14 protein, 31.25 ng
Lane 5 : Recombinant mouse LIGHT/TNFSF14 protein, 15.625 ng
Lane 6 : Recombinant mouse LIGHT/TNFSF14 protein, 7.8 ng
Lane 7 : Recombinant mouse LIGHT/TNFSF14 protein, 3.9 ng
Lane 8 : Recombinant mouse LIGHT/TNFSF14 protein, 1.95 ng
Lane 9 : Recombinant mouse LIGHT/TNFSF14 protein, 0.98 ng
Lane 10 : Recombinant mouse LIGHT/TNFSF14 protein, 0.49 ng
Lane 11 : Recombinant mouse LIGHT/TNFSF14 protein, 0.24 ng

Predicted band size: 26 kDa

Non-reducing conditions..
Sandwich ELISA - Anti-LIGHT/TNFSF14 antibody (ab233502)

ab233502, in conjunction a Biotinylated Anti-Murine LIGHT/TNFSF14 as a detection antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Murine LIGHT/TNFSF14.

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