Biotin Anti-LIGHT/TNFSF14 antibody (ab233504)
Key features and details
- Biotin Rabbit polyclonal to LIGHT/TNFSF14
- Suitable for: WB, Sandwich ELISA
- Reacts with: Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-LIGHT/TNFSF14 antibody
See all LIGHT/TNFSF14 primary antibodies -
Description
Biotin Rabbit polyclonal to LIGHT/TNFSF14 -
Host species
Rabbit -
Conjugation
Biotin -
Tested Applications & Species
See all applications and species dataApplication Species WB Recombinant fragment
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Immunogen
Recombinant fragment corresponding to Mouse LIGHT/TNFSF14 aa 50 to the C-terminus. (E.coli derived).
Database link: Q9QYH9 -
Positive control
- WB: Recombinant mouse LIGHT/TNFSF14 protein.
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General notes
This product was previously labelled as LIGHT
Properties
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Form
Lyophilized:For lot specific reconstitution information please contact our Scientific Support Team. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Constituent: PBS
Sterile filtered -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab233504 was purified by affinity chromatography and then biotinylated. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Biotin Anti-LIGHT/TNFSF14 antibody (ab233504)All lanes : Biotin Anti-LIGHT/TNFSF14 antibody (ab233504) at 0.2 µg/ml
Lane 1 : Recombinant mouse LIGHT/TNFSF14 protein, 250 ng
Lane 2 : Recombinant mouse LIGHT/TNFSF14 protein, 125 ng
Lane 3 : Recombinant mouse LIGHT/TNFSF14 protein, 62.5 ng
Lane 4 : Recombinant mouse LIGHT/TNFSF14 protein, 31.25 ng
Lane 5 : Recombinant mouse LIGHT/TNFSF14 protein, 15.625 ng
Lane 6 : Recombinant mouse LIGHT/TNFSF14 protein, 7.8 ng
Lane 7 : Recombinant mouse LIGHT/TNFSF14 protein, 3.9 ng
Lane 8 : Recombinant mouse LIGHT/TNFSF14 protein, 1.95 ng
Lane 9 : Recombinant mouse LIGHT/TNFSF14 protein, 0.98 ng
Lane 10 : Recombinant mouse LIGHT/TNFSF14 protein, 0.49 ng
Lane 11 : Recombinant mouse LIGHT/TNFSF14 protein, 0.24 ng
Predicted band size: 26 kDaReduced.
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Western blot - Biotin Anti-LIGHT/TNFSF14 antibody (ab233504)All lanes : Biotin Anti-LIGHT/TNFSF14 antibody (ab233504) at 0.2 µg/ml
Lane 1 : Recombinant mouse LIGHT/TNFSF14 protein, 250 ng
Lane 2 : Recombinant mouse LIGHT/TNFSF14 protein, 125 ng
Lane 3 : Recombinant mouse LIGHT/TNFSF14 protein, 62.5 ng
Lane 4 : Recombinant mouse LIGHT/TNFSF14 protein, 31.25 ng
Lane 5 : Recombinant mouse LIGHT/TNFSF14 protein, 15.625 ng
Lane 6 : Recombinant mouse LIGHT/TNFSF14 protein, 7.8 ng
Lane 7 : Recombinant mouse LIGHT/TNFSF14 protein, 3.9 ng
Lane 8 : Recombinant mouse LIGHT/TNFSF14 protein, 1.95 ng
Lane 9 : Recombinant mouse LIGHT/TNFSF14 protein, 0.98 ng
Lane 10 : Recombinant mouse LIGHT/TNFSF14 protein, 0.48 ng
Lane 11 : Recombinant mouse LIGHT/TNFSF14 protein, 0.24 ng
Predicted band size: 26 kDaNon-reducing.
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Sandwich ELISA - Biotin Anti-LIGHT/TNFSF14 antibody (ab233504)ab233504, in conjunction with Anti-Murine LIGHT/TNFSF14 as a capture antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Murine LIGHT/TNFSF14.
