Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LCAT antibody [EPR1384Y] - BSA and Azide free (ab271838)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1384Y] to LCAT - BSA and Azide free
  • Suitable for: IHC-P, IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-LCAT antibody [EPR1384Y] - BSA and Azide free
    See all LCAT primary antibodies

  • Description

    Rabbit monoclonal [EPR1384Y] to LCAT - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IP, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human brain and liver tissue IP: Human plasma

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    ab271838 is the carrier-free version of ab51060. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LCAT antibody [EPR1384Y] - BSA and Azide free (ab271838)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LCAT antibody [EPR1384Y] - BSA and Azide free (ab271838)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling LCAT with purified ab51060 at 1/100 dilution (1.03 µg/mL). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51060).
  • Immunoprecipitation - Anti-LCAT antibody [EPR1384Y] - BSA and Azide free (ab271838)
    Immunoprecipitation - Anti-LCAT antibody [EPR1384Y] - BSA and Azide free (ab271838)

    ab51060 (Purified) at 1/20 dilution (0.5ug) immunoprecipitating LCAT in Human plasma.
    Lane 1 (input): Human plasma 10ug
    Lane 2 (+): ab51060 & Human plasma
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab51060 in Human plasma
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    50 kDa band in input lane should be human IgG heavy chain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51060).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LCAT antibody [EPR1384Y] - BSA and Azide free (ab271838)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LCAT antibody [EPR1384Y] - BSA and Azide free (ab271838)

    ab51060 (unpurified) at 1/100 dilution staining LCAT in paraffin embedded human brain tissue by Immunohistochemistry. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51060).
  • Anti-LCAT antibody [EPR1384Y] - BSA and Azide free (ab271838)
    Anti-LCAT antibody [EPR1384Y] - BSA and Azide free (ab271838)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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