Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LCAT antibody [EPR1384Y] (ab51060)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1384Y] to LCAT
  • Suitable for: IP, WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-LCAT antibody [EPR1384Y]
    See all LCAT primary antibodies

  • Description

    Rabbit monoclonal [EPR1384Y] to LCAT

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human LCAT (C terminal). The exact sequence is proprietary.
    Database link: P04180

  • Positive control

    • WB: Human serum and plasma lysates IHC-P: Human brain and liver tissue IP: Human plasma

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-LCAT antibody [EPR1384Y] (ab51060)
    Western blot - Anti-LCAT antibody [EPR1384Y] (ab51060)
    Anti-LCAT antibody [EPR1384Y] (ab51060) at 1/10000 dilution (Purified) + Human plasma lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 50 kDa
    Observed band size: 62 kDa
    why is the actual band size different from the predicted?



    Blocking/Diluting Buffer and concentration: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LCAT antibody [EPR1384Y] (ab51060)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LCAT antibody [EPR1384Y] (ab51060)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling LCAT with purified ab51060 at 1/100 dilution (1.03 µg/mL). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Western blot - Anti-LCAT antibody [EPR1384Y] (ab51060)
    Western blot - Anti-LCAT antibody [EPR1384Y] (ab51060)
    Anti-LCAT antibody [EPR1384Y] (ab51060) at 1/10000 dilution (unpurified) + Human serum at 10 µg

    Secondary
    Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 50 kDa
    Observed band size: 62 kDa why is the actual band size different from the predicted?

  • Immunoprecipitation - Anti-LCAT antibody [EPR1384Y] (ab51060)
    Immunoprecipitation - Anti-LCAT antibody [EPR1384Y] (ab51060)

    ab51060 (Purified) at 1/20 dilution (0.5ug) immunoprecipitating LCAT in Human plasma.
    Lane 1 (input): Human plasma 10ug
    Lane 2 (+): ab51060 & Human plasma
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab51060 in Human plasma
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    50 kDa band in input lane should be human IgG heavy chain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LCAT antibody [EPR1384Y] (ab51060)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LCAT antibody [EPR1384Y] (ab51060)

    ab51060 (unpurified) at 1/100 dilution staining LCAT in paraffin embedded human brain tissue by Immunohistochemistry. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Anti-LCAT antibody [EPR1384Y] (ab51060)
    Anti-LCAT antibody [EPR1384Y] (ab51060)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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