Anti-6-Nitrotryptophan antibody [2D12] (ab243065)
![Anti-6-Nitrotryptophan antibody [2D12] (ab243065)](https://www.abcam.com/ps/products/243/ab243065/Images/ab243065-332383-5a.png "Anti-6-Nitrotryptophan antibody [2D12] (ab243065)")
Key features and details
- Mouse monoclonal [2D12] to 6-Nitrotryptophan
- Suitable for: WB, Flow Cyt
- Reacts with: Species independent
- Isotype: IgG1
Overview
-
Product name
Anti-6-Nitrotryptophan antibody [2D12]
See all 6-Nitrotryptophan primary antibodies -
Description
Mouse monoclonal [2D12] to 6-Nitrotryptophan -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to 6-Nitrotryptophan conjugated to keyhole limpet haemocyanin.
-
Positive control
- WB: 6-Nitrotryptophan-BSA. Flow cyt: SH-SY5Y cells (treated with hydrogen peroxide).
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
2D12 -
Isotype
IgG1
Images
-
Western blot - Anti-6-Nitrotryptophan antibody [2D12] (ab243065)All lanes : Anti-6-Nitrotryptophan antibody [2D12] (ab243065) at 1/1000 dilution
Lane 1 : BSA at 0.5 µg
Lane 2 : BSA at 1 µg
Lane 3 : 6-Nitrotryptophan-BSA at 0.5 µg
Lane 4 : 6-Nitrotryptophan-BSA at 1 µg
Lane 5 : 7-Ketocholesterol-BSA at 0.5 µg
Lane 6 : 7-Ketocholesterol-BSA at 1 µg
Secondary
All lanes : Goat Anti-Mouse IgG: HRP at 1/2000 dilution
Exposure time: 1 minutePrimary incubation for 2 hours at room temperature.
Secondary incubation for 1 hr at RT.
Block: 5% Skimmed milk in TBST.
-
![Flow Cytometry - Anti-6-Nitrotryptophan antibody [2D12] (ab243065)](https://www.abcam.com/ps/products/243/ab243065/Images/ab243065-332384-65b.png)
Flow Cytometry - Anti-6-Nitrotryptophan antibody [2D12] (ab243065)Flow cytometric analysis of 90% methanol-fixed SH-SY5Y (human neuroblastoma cell line from bone marrow) cells (+/- 250 μM hydrogen peroxide treatment for 24 hours) labeling 6-Nitrotryptophan with ab243065 at 1/50 dilution for 30 minutes on ice compared with a Isotype control IgG and an unlabeled control. A Goat Anti-Mouse: PE at 1/100 dilution was used as the secondary antibody.
