Anti-6-Nitrotryptophan antibody [4F8] (ab243072)
![Anti-6-Nitrotryptophan antibody [4F8] (ab243072)](https://www.abcam.com/ps/products/243/ab243072/Images/ab243072-451405-anti-nitrotryptophan-antibody-4f8-immunohistochemistry-thyroid-human.jpg "Anti-6-Nitrotryptophan antibody [4F8] (ab243072)")
Key features and details
- Mouse monoclonal [4F8] to 6-Nitrotryptophan
- Suitable for: WB, IHC
- Reacts with: Species independent
- Isotype: IgG2b
Overview
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Product name
Anti-6-Nitrotryptophan antibody [4F8]
See all 6-Nitrotryptophan primary antibodies -
Description
Mouse monoclonal [4F8] to 6-Nitrotryptophan -
Host species
Mouse -
Tested applications
Suitable for: WB, IHCmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to 6-Nitrotryptophan conjugated to keyhole limpet haemocyanin.
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Positive control
- WB: 6-Nitrotryptophan-BSA. IHC: Human thyroid cancer tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
4F8 -
Isotype
IgG2b
Images
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Immunohistochemistry - Anti-6-Nitrotryptophan antibody [4F8] (ab243072)
Immunohistochemical analysis of human thryoid cancer tissue labeling 6-Nitrotryptophan with ab243072 (green). Tissue was incubated with ab243072 at 1/100 overnight at 4oC then for 30 minutes at 37oC, followed by incubation with a Goat anti-Mouse IgG (H+L):FITC secondary antibody for 45 minutes at 37oC. Counterstain used was DAPI for 3 minutes at RT. Magnification: 5X.
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![Western blot - Anti-6-Nitrotryptophan antibody [4F8] (ab243072)](https://www.abcam.com/ps/products/243/ab243072/Images/ab243072-332314-anti-6-nitrotryptophan-antibody-4f8-western-blot.png)
Western blot - Anti-6-Nitrotryptophan antibody [4F8] (ab243072)All lanes : Anti-6-Nitrotryptophan antibody [4F8] (ab243072) at 1/1000 dilution
Lane 1 : BSA at 0.5 µg
Lane 2 : BSA at 1 µg
Lane 3 : 6-Nitrotryptophan-BSA at 0.5 µg
Lane 4 : 6-Nitrotryptophan-BSA at 1 µg
Lane 5 : 7-Ketocholesterol-BSA at 0.5 µg
Lane 6 : 7-Ketocholesterol-BSA at 1 µg
Secondary
All lanes : Goat Anti-Mouse IgG: HRP at 1/2000 dilution
Exposure time: 1 minutePrimary incubation for 2 hours at room temperature.
Secondary incubation for 1 hour at room temperature.
Block: 5% Skimmed milk in TBST.
