Anti-CRISPR-associated endonuclease Cas12a antibody [3D3-F7] - BSA and Azide free (ab273478)
![Anti-CRISPR-associated endonuclease Cas12a antibody [3D3-F7] - BSA and Azide free (ab273478)](https://www.abcam.com/ps/products/273/ab273478/Images/ab273478-454978-anti-crisprassociated-endonuclease-cas12a-antibody-3d3f7-western-blot-hek293t.jpg "Anti-CRISPR-associated endonuclease Cas12a antibody [3D3-F7] - BSA and Azide free (ab273478)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [3D3-F7] to CRISPR-associated endonuclease Cas12a - BSA and Azide free
- Suitable for: WB
Overview
-
Product name
Anti-CRISPR-associated endonuclease Cas12a antibody [3D3-F7] - BSA and Azide free
See all CRISPR-associated endonuclease Cas12a primary antibodies -
Description
Mouse monoclonal [3D3-F7] to CRISPR-associated endonuclease Cas12a - BSA and Azide free -
Host species
Mouse -
Specificity
Reacts with Acidaminococcus sp.
-
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHumanRecombinant fragment
-
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: HEK-293T transfected with AsCpf1 Cas12a expression vector containing a myc-His-tag®, whole cell lysate.
-
General notes
ab273478 is the carrier-free version of ab273438. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab273478 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
3D3-F7 -
Isotype
IgG2a
Images
-
Western blot - Anti-CRISPR-associated endonuclease Cas12a antibody [3D3-F7] - BSA and Azide free (ab273478)All lanes : Anti-CRISPR-associated endonuclease Cas12a antibody [3D3-F7] (ab273438) at 1/1000 dilution
Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
Lane 2 : HEK-293T transfected with AsCpf1 Cas12a expression vector containing a myc-His-tag®, whole cell lysate
Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 5 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Exposure time: 3 secondsThis image was produced using ab273438, the same clone but in a different formulation.
Blocking/Dilution buffer: 5% NFDM/TBST.
-
![Anti-CRISPR-associated endonuclease Cas12a antibody [3D3-F7] - BSA and Azide free (ab273478)](https://www.abcam.com/ps/products/273/ab273478/Images/ab273478-455160-benefitsofrecombinantantibodies.jpg)
Anti-CRISPR-associated endonuclease Cas12a antibody [3D3-F7] - BSA and Azide free (ab273478)
