Anti-Laminin beta 2 antibody [EPR23568-91] (ab277521)
![Anti-Laminin beta 2 antibody [EPR23568-91] (ab277521)](https://www.abcam.com/ps/products/277/ab277521/Images/ab277521-423588-anti-laminin-subunit-beta2-antibody-epr2356891-western-blot-lysate.jpg "Anti-Laminin beta 2 antibody [EPR23568-91] (ab277521)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23568-91] to Laminin beta 2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Laminin beta 2 antibody [EPR23568-91]
See all Laminin beta 2 primary antibodies -
Description
Rabbit monoclonal [EPR23568-91] to Laminin beta 2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human heart, colon, skeletal muscle and placenta tissue lysates; mouse heart tissue lysate; rat heart tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23568-91 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Laminin beta 2 antibody [EPR23568-91] (ab277521)All lanes : Anti-Laminin beta 2 antibody [EPR23568-91] (ab277521) at 1/1000 dilution
Lane 1 : Human heart tissue lysate
Lane 2 : Human colon tissue lysate
Lane 3 : Human skeletal muscle tissue lysate
Lane 4 : Human placenta tissue lysate
Lane 5 : Human liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution
Predicted band size: 196 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The observed MW is consistent with what has been described in the literature (PMID: 9273822 ).
Low expression: human liver (PMID:7795887).
Exposure time: Lane 1: 48 seconds
Lane 2-5: 3minutes.
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![Western blot - Anti-Laminin beta 2 antibody [EPR23568-91] (ab277521)](https://www.abcam.com/ps/products/277/ab277521/Images/ab277521-423589-anti-laminin-subunit-beta2-antibody-epr2356891-western-blot-lysate1.jpg)
Western blot - Anti-Laminin beta 2 antibody [EPR23568-91] (ab277521)All lanes : Anti-Laminin beta 2 antibody [EPR23568-91] (ab277521) at 1/1000 dilution
Lane 1 : Mouse heart tissue lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Rat heart tissue lysate
Lane 4 : Rat liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 196 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The observed MW is consistent with what has been described in the literature (PMID: 9273822).
Low expression: mouse liver, rat liver (PMID: 7795887)
Exposure time: Lane 1, 2, 4: 3minutes
Lane 3: 70 seconds.
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![Anti-Laminin beta 2 antibody [EPR23568-91] (ab277521)](https://www.abcam.com/ps/products/277/ab277521/Images/ab277521-447372-benefitsofrecombinantantibodies.png)
Anti-Laminin beta 2 antibody [EPR23568-91] (ab277521)