Human Osteopontin ELISA Kit (ab100618)
Key features and details
- Sensitivity: 50 pg/ml
- Range: 74.07 pg/ml - 18000 pg/ml
- Sample type: Cell culture supernatant, Milk, Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 500X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human Osteopontin (lyophilized) 2 vials Osteopontin Microplate (12 x 8 wells) 1 unit Recombinant Human Osteopontin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
-
Function
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. -
Tissue specificity
Bone. Found in plasma. -
Sequence similarities
Belongs to the osteopontin family. -
Post-translational
modificationsExtensively phosphorylated on clustered serine residues.
N- and O-glycosylated.
Phosphorylation sites are present in the extracelllular medium. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- BNSP
- Bone sialoprotein 1
- Bone sialoprotein I
see all -
Database links
- Entrez Gene: 6696 Human
- Omim: 166490 Human
- SwissProt: P10451 Human
- Unigene: 313 Human
Images
-
Sandwich ELISA - Osteopontin Human ELISA Kit (ab100618)
Osteopontin measured in biological fluids showing quantity (pg) per mL of tested sample. Human serum diluted 10-30 fold. Human plasma diluted 50 fold - below detection. Human milk diluted 18000-54000 fold. Human urine diluted 100 fold.
-
Typical Standard CurveRepresentative Standard Curve using ab100618.
-
Typical Standard CurveRepresentative Standard Curve using ab100618.