Anti-Lamin A + Lamin C antibody (ab224816)
Key features and details
- Rabbit polyclonal to Lamin A + Lamin C
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-Lamin A + Lamin C antibody
See all Lamin A + Lamin C primary antibodies -
Description
Rabbit polyclonal to Lamin A + Lamin C -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Pig
-
Immunogen
Synthetic peptide within Human Lamin A + Lamin C aa 1-50. The exact sequence is proprietary. (NP_733821.1).
Database link: P02545 -
Positive control
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human colon carcinoma tissue; Mouse teratoma tissue. ICC/IF: HeLa cells.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab224816 was affinity purified using an epitope specific to Lamin A + C immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Lamin A + Lamin C antibody (ab224816)All lanes : Anti-Lamin A + Lamin C antibody (ab224816) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 74 kDa
Exposure time: 1 second
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody (ab224816)Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for Lamin A + C using ab224816 at 1/200 (left panel) and 1/1000 (right panel) dilution in immunohistochemical analysis. Detection: DAB staining.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody (ab224816)Formalin-fixed, paraffin-embedded mouse teratoma tissue stained for Lamin A + C using ab224816 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining.
-
Immunoprecipitation - Anti-Lamin A + Lamin C antibody (ab224816)Lamin A + C was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab224816 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab224816 at 0.4 µg/ml.
Lane 1: ab224816 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 1 second. -
Immunocytochemistry/ Immunofluorescence - Anti-Lamin A + Lamin C antibody (ab224816)Formaldehyde-fixed asynchronous HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Lamin A + C using ab224816 at 1/200 dilution in ICC/IF. Detection: Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at 1/100 dilution.
