Anti-Lamin A antibody (ab226198)
Key features and details
- Rabbit polyclonal to Lamin A
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Lamin A antibody
See all Lamin A primary antibodies -
Description
Rabbit polyclonal to Lamin A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Pig
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Immunogen
Synthetic peptide within Human Lamin A aa 550-600. The exact sequence is proprietary. (NP_733821.1).
Database link: P02545 -
Positive control
- WB: HeLa and NIH/3T3 whole cell lysates. IHC-P: Human testicular seminoma tissue; Mouse teratoma tissue. IP: HeLa whole cell lysate (ab150035).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226198 was affinity purified using an epitope specific to Lamin A immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Lamin A antibody (ab226198)All lanes : Anti-Lamin A antibody (ab226198) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 74 kDa
Exposure time: 1 second
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A antibody (ab226198)Formalin-fixed, paraffin-embedded human testicular seminoma tissue stained for Lamin A using ab226198 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.
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Immunoprecipitation - Anti-Lamin A antibody (ab226198)Lamin A was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226198 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226198 at 0.4 µg/ml.
Lane 1: ab226198 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 1 second.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A antibody (ab226198)Formalin-fixed, paraffin-embedded mouse teratoma tissue stained for Lamin A using ab226198 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining.
