Anti-KDM3B / JMJD1B antibody (ab70797)
Key features and details
- Rabbit polyclonal to KDM3B / JMJD1B
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KDM3B / JMJD1B antibody -
Description
Rabbit polyclonal to KDM3B / JMJD1B -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus -
Immunogen
Synthetic peptide corresponding to Human KDM3B/ JMJD1B (C terminal).
Database link: Q7LBC6 -
Positive control
- HeLa and 293T whole cell lysates. IHC Controls: Laryngeal squamous cell carcinoma, Ovarian carcinoma, Prostate carcinoma
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General notes
The immunogen used for this product shares 76% homology with KDM3A/JMJD1A. Cross-reactivity with this protein has not been confirmed experimentally.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab70797 was affinity purified using an epitope specific to KDM3B / JMJD1B immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-KDM3B / JMJD1B antibody (ab70797) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 191 kDa
Observed band size: 240 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa, 30 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM3B / JMJD1B antibody (ab70797)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling KDM3B / JMJD1B with ab70797 at 1/200 (1µg/ml). Detection: DAB.
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Detection of KDM3B / JMJD1B by Western Blot of Immunoprecipitate.
ab70797 at 1µg/ml staining KDM3B / JMJD1B in HeLa whole cell lysate immunoprecipitated using ab70797 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.