Anti-IRR antibody [EP327Y] (ab40782)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP327Y] to IRR
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
Overview
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Product name
Anti-IRR antibody [EP327Y]
See all IRR primary antibodies -
Description
Rabbit monoclonal [EP327Y] to IRR -
Host species
Rabbit -
Specificity
ab40782 recognises insulin receptor related receptor (IRR). -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Rat -
Immunogen
Synthetic peptide within Human IRR aa 1-100 (N terminal). The exact sequence is proprietary.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP327Y -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-IRR antibody [EP327Y] (ab40782) at 1/2000 dilution
Lane 1 : PC12
Lane 2 : MCF7
Lane 3 : Hela
Lane 4 : SHSY5Y
Lysates/proteins at 10 µg per lane.
Predicted band size: 80, 144 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Additional bands at: 130 kDa, 37 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemical analysis of paraffin-embedded human kidney tissue using ab40782.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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