Anti-EGFR (phospho T669) antibody [EP2256Y] (ab75980)
![Anti-EGFR (phospho T669) antibody [EP2256Y] (ab75980)](https://www.abcam.com/ps/products/75/ab75980/Images/ab75980-256540-anti-egfr-phospho-t669-antibody-ep2256y-western-blot.jpg "Anti-EGFR (phospho T669) antibody [EP2256Y] (ab75980)")
Key features and details
- Rabbit monoclonal [EP2256Y] to EGFR (phospho T669)
- Suitable for: ICC/IF, Dot blot, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-EGFR (phospho T669) antibody [EP2256Y]
See all EGFR primary antibodies -
Description
Rabbit monoclonal [EP2256Y] to EGFR (phospho T669) -
Host species
Rabbit -
Specificity
Recognises EGFR phosphorylated on Threonine 669 of the mature human isoform 1 (corresponding to T693 from the precursor form P00533-1/p170)
-
Tested applications
Suitable for: ICC/IF, Dot blot, WBmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: A431 and A431 treated with EGF. ICC: A431 cells.
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP2256Y -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-EGFR (phospho T669) antibody [EP2256Y] (ab75980)All lanes : Anti-EGFR (phospho T669) antibody [EP2256Y] (ab75980) at 1/20000 dilution
Lane 1 : A431 (human epidermoid carcinoma) treated with Epidermal Growth Factor (EGF) whole cell lysates
Lane 2 : Untreated A431 (human epidermoid carcinoma) whole cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Predicted band size: 134 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking and dilution buffer: 5% NFDM/TBST
-
![Dot Blot - Anti-EGFR (phospho T669) antibody [EP2256Y] (ab75980)](https://www.abcam.com/ps/products/75/ab75980/Images/ab75980-256541-anti-egfr-phospho-t669-antibody-ep2256y-dot-blot.jpg)
Dot Blot - Anti-EGFR (phospho T669) antibody [EP2256Y] (ab75980)Primary antibody dilution: 1/1000
Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated
Secondary antibody dilution: 1/2500
Blocking & dilution buffer: 5% NFDM/TBST
Lane 1 sample: EGFR (pT669) phospho peptide
Lane 2 sample: EGFR non-phospho peptide
Exposure time: 3 minutes
-
![Western blot - Anti-EGFR (phospho T669) antibody [EP2256Y] (ab75980)](https://www.abcam.com/ps/products/75/ab75980/Images/ab75980-91213-anti-egfr-phospho-t669-antibody-ep2256y-western-blot.gif)
Western blot - Anti-EGFR (phospho T669) antibody [EP2256Y] (ab75980)All lanes : Anti-EGFR (phospho T669) antibody [EP2256Y] (ab75980) at 1/20000 dilution
Lane 1 : A431 cell lysate, untreated
Lane 2 : A431 cell lysate, treated with EGF
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 134 kDa
Observed band size: 175 kDa why is the actual band size different from the predicted?
-
![Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho T669) antibody [EP2256Y] (ab75980)](https://www.abcam.com/ps/products/75/ab75980/Images/ab75980-91212-anti-egfr-phospho-t669-antibody-ep2256y-immunofluorescence.gif)
Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho T669) antibody [EP2256Y] (ab75980)ab75980 at 1/100 dilution staining EGFR in A431 cells by Immunofluorescence.
