Anti-NSE antibody - Neuronal Marker (ab53025)
Key features and details
- Rabbit polyclonal to NSE - Neuronal Marker
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NSE antibody - Neuronal Marker
See all NSE primary antibodies -
Description
Rabbit polyclonal to NSE - Neuronal Marker -
Host species
Rabbit -
Specificity
This antibody detects endogenous levels of total NSE protein. -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide derived from human NSE
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NSE antibody - Neuronal Marker (ab53025)All lanes : Anti-NSE antibody - Neuronal Marker (ab53025) at 1/500 dilution
Lane 1 : Extracts from HepG2 cells with no immunizing peptide
Lane 2 : Extracts from HepG2 cells with immunizing peptide
Predicted band size: 47 kDa
Observed band size: 47 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-NSE antibody - Neuronal Marker (ab53025)ICC/IF image of ab53025 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53025, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody - Neuronal Marker (ab53025)Immunohistochemical analysis of paraffin-embedded human brain tissue using ab53025 at 1/50-1/100 dilution. Left: without immunizing peptide; Right: with immunizing peptide -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody - Neuronal Marker (ab53025) Image from Yu Yang. et. al. Mol. Cell. Proteomics, Mar 2009 (Fig 5).ab53025 staining NSE in mouse brain tissue section by Immunohistochemistry (PFA-perfussion fixed frozen tissue sections). Tissues were collected from mice in each group at 10 weeks. Mice were euthanized, the brains were exposed and removed from the body. Then the brain was cut, on ice and fixed in 4% polyformaldehyde or glutaraldehyde solution for histological examination. The primary antibody was used at 1/75 dilution in PBS and incubated with sample for 2 hours at 37°C. A HRP-conjugated secondary antibody was used at for 1hr at 37°C in a humidity box.
