Anti-GM-CSF antibody [EPR23689-17] (ab227031)
![Anti-GM-CSF antibody [EPR23689-17] (ab227031)](https://www.abcam.com/ps/products/227/ab227031/Images/ab227031-1-anti-gmcsf-antibody-epr2368917-flow-cytometry-human-peripheral-blood-mononuclear-cell.jpg "Anti-GM-CSF antibody [EPR23689-17] (ab227031)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23689-17] to GM-CSF
- Suitable for: Flow Cyt (Intra)
- Reacts with: Mouse, Human
Overview
-
Product name
Anti-GM-CSF antibody [EPR23689-17]
See all GM-CSF primary antibodies -
Description
Rabbit monoclonal [EPR23689-17] to GM-CSF -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt MouseHuman
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- Flow Cyt: Human peripheral blood mononuclear cell (PBMC) treated with cell stimulation cocktail (80nM PMA+1.34uM Ionomycin+10.6uM Brefeldin A+2uM Monensin) for 6 hours; Mouse peripheral blood mononuclear cell (PBMC) treated with cell stimulation cocktail (80nM PMA+1.34uM Ionomycin+10.6uM Brefeldin A+2uM Monensin) for 6 hours.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23689-17 -
Isotype
IgG1 -
Research areas
Images
-
Flow Cytometry - Anti-GM-CSF antibody [EPR23689-17] (ab227031)
Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Human peripheral blood mononuclear cell (PBMC) treated with cell stimulation cocktail (80nM PMA+1.34uM Ionomycin+10.6uM Brefeldin A+2uM Monensin) for 6 hours cells labelling GM-CSF with ab227031 at 1/50 dilution Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were stained with anti-CD4 conjugated to Alexa Fluor® 647. Then fixed with 2% PFA followed by intracellularly stained with rabbit IgG or ab227031.
-
![Flow Cytometry - Anti-GM-CSF antibody [EPR23689-17] (ab227031)](https://www.abcam.com/ps/products/227/ab227031/Images/ab227031-2-anti-gmcsf-antibody-epr2368917-flow-cytometry-mouse-peripheral-blood-mononuclear-cell.jpg)
Flow Cytometry - Anti-GM-CSF antibody [EPR23689-17] (ab227031)Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Mouse peripheral blood mononuclear cell (PBMC) treated with cell stimulation cocktail (80nM PMA+1.34uM Ionomycin+10.6uM Brefeldin A+2uM Monensin) for 6 hours cells labelling GM-CSF with ab227031 at 1/50 dilution Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were stained with anti-CD4 conjugated to Alexa Fluor® 647. Then fixed with 2% PFA followed by intracellularly stained with rabbit IgG or ab227031.