Anti-IRF6 antibody [OTI2A12] (ab123880)
![Anti-IRF6 antibody [OTI2A12] (ab123880)](https://www.abcam.com/ps/products/123/ab123880/Images/IRF6-Primary-antibodies-ab123880-1.jpg "Anti-IRF6 antibody [OTI2A12] (ab123880)")
Key features and details
- Mouse monoclonal [OTI2A12] to IRF6
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG2b
Overview
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Product name
Anti-IRF6 antibody [OTI2A12]
See all IRF6 primary antibodies -
Description
Mouse monoclonal [OTI2A12] to IRF6 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF African green monkeyIHC-P HumanWB Human
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Immunogen
Recombinant full length protein corresponding to Human IRF6. Produced in HEK293 cell (NP_006138)
Database link: O14896 -
Positive control
- Purchase matching WB positive control:Recombinant Human IRF6 protein
- WB: HEK293T cells transfected with pCMV6-ENTRY IRF6 cDNA IHC-P: adenocarcinoma of Human colon tissue, Human kidney tissue, carcinoma of Human lung tissue, adenocarcinoma of Human endometrium tissue ICC/IF: COS7 cells transiently transfected by pCMV6-ENTRY IRF6 Flow Cyt: HEK293T cells transfected with pCMV6-ENTRY IRF6 overexpress plasmid
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 1% BSA, PBS -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant by affinity chromatography. -
Clonality
Monoclonal -
Clone number
OTI2A12 -
Isotype
IgG2b -
Research areas
Images
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Western blot - Anti-IRF6 antibody [OTI2A12] (ab123880)All lanes : Anti-IRF6 antibody [OTI2A12] (ab123880) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY IRF6 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 53 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF6 antibody [OTI2A12] (ab123880)](https://www.abcam.com/ps/products/123/ab123880/Images/IRF6-Primary-antibodies-ab123880-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF6 antibody [OTI2A12] (ab123880)Paraffin-embedded human colon adenocarcinoma tissue stained for IRF6 using ab123880 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF6 antibody [OTI2A12] (ab123880)](https://www.abcam.com/ps/products/123/ab123880/Images/IRF6-Primary-antibodies-ab123880-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF6 antibody [OTI2A12] (ab123880)Paraffin-embedded human kidney tissue stained for IRF6 using ab123880 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF6 antibody [OTI2A12] (ab123880)](https://www.abcam.com/ps/products/123/ab123880/Images/IRF6-Primary-antibodies-ab123880-4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF6 antibody [OTI2A12] (ab123880)Paraffin-embedded human lung carcinoma tissue stained for IRF6 using ab123880 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF6 antibody [OTI2A12] (ab123880)](https://www.abcam.com/ps/products/123/ab123880/Images/IRF6-Primary-antibodies-ab123880-5.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF6 antibody [OTI2A12] (ab123880)Paraffin-embedded human endometrium adenocarcinoma tissue stained for IRF6 using ab123880 at 1/150 dilution in immunohistochemical analysis.
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![Immunocytochemistry/ Immunofluorescence - Anti-IRF6 antibody [OTI2A12] (ab123880)](https://www.abcam.com/ps/products/123/ab123880/Images/IRF6-Primary-antibodies-ab123880-7.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-IRF6 antibody [OTI2A12] (ab123880)COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected using pCMV6-ENTRY IRF6 stained for IRF6 (green) in ICC/IF.
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![Flow Cytometry - Anti-IRF6 antibody [OTI2A12] (ab123880)](https://www.abcam.com/ps/products/123/ab123880/Images/IRF6-Primary-antibodies-ab123880-8.jpg)
Flow Cytometry - Anti-IRF6 antibody [OTI2A12] (ab123880)Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either pCMV6-ENTRY IRF6 overexpress plasmid (Red), or empty vector control plasmid (Blue).